caa a22 4500 606229825 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0108-z doi (NATIONALLICENCE)springer-10.1007/s10695-015-0108-z Identification of embryonic stem cell activities in an embryonic cell line derived from marine medaka ( Oryzias dancena ) [Elektronische Daten] [Dongwook Lee, Jun Ryu, Seung Lee, Yoon Nam, Dong Kim, Seung Gong] This study was conducted to identify embryonic stem cell (ESC) activities of a long-term cultured embryonic cell line previously derived from blastula-stage Oryzias dancena embryos. Five sub-cell lines were established from the embryonic cell line via clonal expansion of single cells. ESC activities, including clonogenicity, alkaline phosphatase (AP) activity, and differentiation capacity, were examined in the five sub-cell lines. We observed both clonogenicity and AP activity in all five sub-cell lines, but the proportion of cells that exhibited both properties was significantly different among them. Even though we detected different formation rates and sizes of embryoid body (EB) among these cells, all lines were stably able to form EBs and further induction for differentiation showed their capability to differentiate into other cell types in a spontaneous manner. From this study, we determined that the embryonic cell lines examined possessed heterogeneous ESC activities and can be utilized as a marine model system for fish ESC-based research. Springer Science+Business Media Dordrecht, 2015 Marine model nationallicence Oryzias dancena nationallicence Embryonic stem cells nationallicence Heterogeneity nationallicence Lee Dongwook Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut Ryu Jun Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut Lee Seung Department of Animal Life Science, Kangwon National University, 200-701, Chuncheon, Korea aut Nam Yoon Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut Kim Dong Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut Gong Seung Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1569-1576 0920-1742 41:6<1569 2015 41 10695 https://doi.org/10.1007/s10695-015-0108-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0108-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Dongwook Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 700 1- Ryu Jun Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 700 1- Lee Seung Department of Animal Life Science, Kangwon National University, 200-701, Chuncheon, Korea aut NATIONALLICENCE 700 1- Nam Yoon Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 700 1- Kim Dong Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 700 1- Gong Seung Department of Fisheries Biology, Pukyong National University, 608-737, Busan, Korea aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1569-1576 0920-1742 41:6<1569 2015 41 10695