caa a22 4500 606229833 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0096-z doi (NATIONALLICENCE)springer-10.1007/s10695-015-0096-z Molecular identification and expression of the Foxl2 gene during gonadal sex differentiation in northern snakehead Channa argus [Elektronische Daten] [Dan-Dan Wang, Gui-Rong Zhang, Kai-Jian Wei, Wei Ji, Jonathan Gardner, Rui-Bin Yang, Kun-Ci Chen] Channa argus is one of the most commercially important fish species in China. Studies show that males of C. argus grow faster than females at the same age. In order to explore the sex differentiation mechanism of C. argus, we isolated the full length of the sex-related gene Foxl2 cDNA and analysed its expression patterns during gonadal sex differentiation. Alignment of known Foxl2 amino acid sequences from vertebrates confirmed the conservation of the Foxl2 open reading frame, especially the forkhead domain and C-terminal region. Quantitative RT-PCR revealed that Foxl2 is predominantly expressed in brain, pituitary, gill and ovary, with its highest level in ovary but low levels in testis and other tissues, reflecting a potential role for Foxl2 in the brain-pituitary-gonad axis in C. argus. Our ontogenetic stage data showed that C. argus Foxl2 expression was significantly upregulated from 1 to 11days posthatching (dph) and that the initiation of expression preceded the first anatomical ovarian differentiation (27dph), suggesting that Foxl2 might play a potential role in early gonadal sex differentiation in C. argus. In addition, the Foxl2 protein was primarily located in granulosa cells surrounding the oocytes of mature C. argus, implying that Foxl2 may have a basic function in granulosa cell differentiation and the maintenance of oocytes. Springer Science+Business Media Dordrecht, 2015 Foxl2 nationallicence Molecular identification nationallicence Gene expression nationallicence Early gonadal development nationallicence Channa argus nationallicence Wang Dan-Dan Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Zhang Gui-Rong Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Wei Kai-Jian Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Ji Wei Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Gardner Jonathan Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Yang Rui-Bin Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Chen Kun-Ci Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, Guangzhou, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1419-1433 0920-1742 41:6<1419 2015 41 10695 https://doi.org/10.1007/s10695-015-0096-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0096-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Dan-Dan Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Gui-Rong Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Wei Kai-Jian Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Ji Wei Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Gardner Jonathan Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Yang Rui-Bin Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Chen Kun-Ci Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 510380, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1419-1433 0920-1742 41:6<1419 2015 41 10695