caa a22 4500 606229841 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0104-3 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0104-3 Expression and ontogeny of growth hormone (Gh) in the protogynous hermaphroditic ricefield eel ( Monopterus albus ) [Elektronische Daten] [Dong Chen, Jiang Liu, Wanping Chen, Shuxia Shi, Weimin Zhang, Lihong Zhang] Growth hormone (GH) is a single-chain polypeptide hormone mainly secreted by somatotropes of the anterior pituitary gland and is an important regulator of somatic growth in vertebrates including teleosts. In this study, a polyclonal antiserum against ricefield eel Gh was generated and the expression of Gh at the mRNA and protein levels was analyzed. Both RT-PCR and western blot analysis showed that Gh was predominantly expressed in the pituitary glands of ricefield eels. The immunoreactive Gh signals were localized to the multicellular layers of the adenohypophysis adjacent to the neurohypophysis in ricefield eels. Ontogenetic analysis showed that immunoreactive Gh signals could be detected in the pituitary glands of ricefield eel embryos as early as 3days post-fertilization. During the sex change from female to male, the levels of the immunoreactive Gh signals in the pituitary glands of the ricefield eels peaked at the intersexual stage. These results suggest that Gh in the pituitary glands may be associated with embryonic development before hatching, as well as with the sex change in the adult ricefield eels, possibly via the classical endocrine manner. Springer Science+Business Media Dordrecht, 2015 Ricefield eel ( Monopterus albus ) nationallicence Pituitary nationallicence Growth hormone nationallicence Ontogeny nationallicence Sex change nationallicence Chen Dong School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Liu Jiang School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Chen Wanping School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Shi Shuxia School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Zhang Weimin School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Zhang Lihong School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1515-1525 0920-1742 41:6<1515 2015 41 10695 https://doi.org/10.1007/s10695-015-0104-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0104-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Dong School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jiang School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Wanping School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Shi Shuxia School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Weimin School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Lihong School of Life Sciences, Sun Yat-Sen University, 510275, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1515-1525 0920-1742 41:6<1515 2015 41 10695