caa a22 4500 60622985X CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0095-0 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0095-0 Proteomic analysis of early-stage embryos: implications for egg quality in hapuku ( Polyprion oxygeneios ) [Elektronische Daten] [Yair Kohn, Jane Symonds, Torsten Kleffmann, Shinichi Nakagawa, Malgorzata Lagisz, P. Lokman] In order to develop biomarkers that may help predict the egg quality of captive hapuku (Polyprion oxygeneios) and provide potential avenues for its manipulation, the present study (1) sequenced the proteome of early-stage embryos using isobaric tag for relative and absolute quantification analysis, and (2) aimed to establish the predictive value of the abundance of identified proteins with regard to egg quality through regression analysis. Egg quality was determined for eight different egg batches by blastomere symmetry scores. In total, 121 proteins were identified and assigned to one of nine major groups according to their function/pathway. A mixed-effects model analysis revealed a decrease in relative protein abundance that correlated with (decreasing) egg quality in one major group (heat-shock proteins). No differences were found in the other protein groups. Linear regression analysis, performed for each identified protein separately, revealed seven proteins that showed a significant decrease in relative abundance with reduced blastomere symmetry: two correlates that have been named in other studies (vitellogenin, heat-shock protein-70) and a further five new candidate proteins (78kDa glucose-regulated protein, elongation factor-2, GTP-binding nuclear protein Ran, iduronate 2-sulfatase and 6-phosphogluconate dehydrogenase). Notwithstanding issues associated with multiple statistical testing, we conclude that these proteins, and especially iduronate 2-sulfatase and the generic heat-shock protein group, could serve as biomarkers of egg quality in hapuku. Springer Science+Business Media Dordrecht, 2015 Hapuku nationallicence Polyprion oxygeneios nationallicence Egg quality nationallicence Proteomics nationallicence iTRAQ nationallicence Biomarkers nationallicence Kohn Yair Department of Zoology, University of Otago, 340 Great King St., PO Box 56, 9016, Dunedin, New Zealand aut Symonds Jane Bream Bay Aquaculture Park, NIWA, PO Box 147, 0151, Ruakaka, New Zealand aut Kleffmann Torsten Centre for Protein Research, University of Otago, PO Box 56, 9054, Dunedin, New Zealand aut Nakagawa Shinichi Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia aut Lagisz Malgorzata Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia aut Lokman P. Department of Zoology, University of Otago, 340 Great King St., PO Box 56, 9016, Dunedin, New Zealand aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1403-1417 0920-1742 41:6<1403 2015 41 10695 https://doi.org/10.1007/s10695-015-0095-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0095-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kohn Yair Department of Zoology, University of Otago, 340 Great King St., PO Box 56, 9016, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Symonds Jane Bream Bay Aquaculture Park, NIWA, PO Box 147, 0151, Ruakaka, New Zealand aut NATIONALLICENCE 700 1- Kleffmann Torsten Centre for Protein Research, University of Otago, PO Box 56, 9054, Dunedin, New Zealand aut NATIONALLICENCE 700 1- Nakagawa Shinichi Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia aut NATIONALLICENCE 700 1- Lagisz Malgorzata Evolution & Ecology Research Centre and School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia aut NATIONALLICENCE 700 1- Lokman P. Department of Zoology, University of Otago, 340 Great King St., PO Box 56, 9016, Dunedin, New Zealand aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1403-1417 0920-1742 41:6<1403 2015 41 10695