caa a22 4500 606229868 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0109-y doi (NATIONALLICENCE)springer-10.1007/s10695-015-0109-y Changes in digestive enzyme activities during larval development of Chinese loach Paramisgurnus dabryanus (Dabry de Thiersant, 1872) [Elektronische Daten] [Yun-Long Zhang, Qiao-Wan Wu, Wei-Hua Hu, Fan Wang, Zhong-Bo Zhao, Hui He, Wei-Han Shao, Qi-Xue Fan] The digestive physiology of Chinese loach (Paramisgurnus dabryanus) was studied by assessing the specific and total activities of different pancreatic (trypsin, chymotrypsin, amylase and lipase), gastric (pepsin) and intestinal (alkaline phosphatase and leucine-aminopeptidase) enzymes from hatching to 40days after hatching (DAH). Larvae were reared at 24.4±0.4°C and fed with rotifers from mouth opening (4 DAH) to 15 DAH, from 10 to 35 DAH with Cladocera and from 30 to 40 DAH with compound diet. Enzyme activities for trypsin, chymotrypsin, amylase and lipase were detected before the onset of exogenous feeding, indicating that these enzymes were genetically pre-programmed. Most of the pancreatic enzyme specific activities increased until 20 DAH and decreased thereafter. The pepsin activity of Chinese loach was firstly detected at 30 DAH, indicating the appearance of functional gastric gland. Alkaline phosphatase specific activity was detected from hatching onward, showed marked increase and reached the second peak at 20 DAH, while a gradual increase in specific leucine-aminopeptidase activity was observed until the end of the experiment. Accordingly, the larvae of Chinese loach possess a functional digestive system before the onset of exogenous feeding and the digestive capacity gradually increases as development progresses. The abrupt increase in intestinal enzyme activities between 10 and 20 DAH demonstrates onset of juvenile-like digestive mode in Chinese loach larvae. The increase in pepsin activity after 30 DAH indicates the shift from alkaline to acidic digestion in Chinese loach larvae, which may be considered as the onset of weaning. Springer Science+Business Media Dordrecht, 2015 Paramisgurnus dabryanus nationallicence Digestive enzyme nationallicence Ontogeny nationallicence Larval development nationallicence Zhang Yun-Long KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Wu Qiao-Wan KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Hu Wei-Hua KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Wang Fan KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Zhao Zhong-Bo KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut He Hui KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Shao Wei-Han KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Fan Qi-Xue KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1577-1585 0920-1742 41:6<1577 2015 41 10695 https://doi.org/10.1007/s10695-015-0109-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0109-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yun-Long KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Wu Qiao-Wan KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Hu Wei-Hua KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Fan KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Zhong-Bo KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- He Hui KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Shao Wei-Han KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Fan Qi-Xue KeyLabofFreshwaterAnimalBreeding,MinistryofAgriculture, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1577-1585 0920-1742 41:6<1577 2015 41 10695