caa a22 4500 606229884 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10695-015-0097-y doi (NATIONALLICENCE)springer-10.1007/s10695-015-0097-y The involvement of gonadotropins and gonadal steroids in the ovulatory dysfunction of the potamodromous Salminus hilarii (Teleostei: Characidae) in captivity [Elektronische Daten] [Renata Moreira, Renato Honji, Renato Melo, Amanda de Moraes Narcizo, Juliane Amaral, Ronaldo de Carvalho Araújo, Alexandre Hilsdorf] Potamodromous teleosts that require migration to reproduce show dysfunctions that block ovulation and spawning while in captivity. To understand the physiological basis of these reproductive dysfunctions, follicle-stimulating hormone b subunit (fshb) and luteinizing hormone b subunit (lhb) gene expression analyses by real-time quantitative PCR, together with measurements of estradiol (E 2), 17α-hydroxyprogesterone (17α-OHP) and 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DHP) levels, were carried out throughout the reproductive cycle of the potamodromous Salminus hilarii. The following reproductive stages were evaluated in captive and wild females: previtellogenic (PV), advanced maturation/mature (AM) and regression/spent (REG/SPENT). In the wild females, fshb expression decreased from the PV to the AM stage, and the opposite pattern was detected for E 2, which increased from the PV to the AM stage. fshb was expressed at lower levels in captive than in wild females, and this difference did not change during the reproductive cycle. lhb expression also increased from the PV to the AM stage in both groups, but the wild females at the AM and REG/SPENT stages showed higher lhb expression levels than the captive females. The concentrations of 17α-OHP did not change during the reproductive cycle, and the levels were higher in the captive than in the wild females at all reproductive stages. 17α,20β-DHP levels did not change between wild and captive females. However, in captive females, the transition from PV to AM stage was followed by an increase in 17α,20β-DHP levels. These data indicate that dysfunctions in the gonadotropins and steroids synthesis pathways cause the ovulation failure in captive S. hilarii. Springer Science+Business Media Dordrecht, 2015 Estradiol nationallicence Gonadotropins nationallicence 17α-Hydroxyprogesterone nationallicence 17α,20β-Dihydroxy-4-pregnen-3-one nationallicence Reproductive dysfunction nationallicence Moreira Renata Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut Honji Renato Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut Melo Renato Núcleo de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil aut de Moraes Narcizo Amanda Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut Amaral Juliane Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut de Carvalho Araújo Ronaldo Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Hilsdorf Alexandre Núcleo de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil aut Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1435-1447 0920-1742 41:6<1435 2015 41 10695 https://doi.org/10.1007/s10695-015-0097-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0097-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moreira Renata Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Honji Renato Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Melo Renato Núcleo de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil aut NATIONALLICENCE 700 1- de Moraes Narcizo Amanda Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Amaral Juliane Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, Travessa 14, no 321, 05508-090, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- de Carvalho Araújo Ronaldo Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Hilsdorf Alexandre Núcleo de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, SP, Brazil aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/6(2015-12-01), 1435-1447 0920-1742 41:6<1435 2015 41 10695