caa a22 4500 606229892 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0032-2 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0032-2 Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish ( Carassius auratus ) exposed to monocrotophos [Elektronische Daten] [Fei Zhao, Bai Wang, Xiaona Zhang, Hua Tian, Wei Wang, Shaoguo Ru] Using goldfish (Carassius auratus) as the model animal, the present study revealed the types of the DNA damage induced by monocrotophos, a highly toxic organophosphorus pesticide, and explored the mechanism underlying the DNA-damaging effect of this pesticide. Results of the alkaline comet assay showed that global DNA damage (including single- and double-strand breaks and alkali-labile sites) in peripheral erythrocytes of goldfish, measured as olive tail moment, was significantly increased by exposure to 0.01, 0.10, and 1.00mg/L monocrotophos for 24, 48, 96, and 168h. In particular, alkali-labile sites rather than single- or double-strand breaks, distinguished by the alkaline, pH 12.1, and neutral comet assays, were mainly induced by monocrotophos at 48h. Oxidative damage in DNA bases and telomeric DNA was investigated by using the alkaline comet assay combined with endonuclease III or formamidopyrimidine DNA glycosylase and with fluorescence in situ hybridization, respectively. Further, glutathione peroxidase activity significantly decreased at 24h but increased at 96 and 168h, and malondialdehyde concentrations significantly increased at 48h but gradually decreased at 96 and 168h, which indicated an over-production of reactive oxygen species (ROS) at short exposure durations, but effective scavenging at long exposure durations in the peripheral blood tissues. Accordingly, our results suggest that DNA damage induced by monocrotophos in fish blood cells is possibly due to the inhibition of ROS scavenging and resulted accumulation of ROS. Springer Science+Business Media Dordrecht, 2015 Carassius auratus nationallicence Monocrotophos nationallicence Comet assay nationallicence Alkali-labile sites nationallicence Strand breaks nationallicence Reactive oxygen species nationallicence ROS : Reactive oxygen species nationallicence SSB : Single-strand breaks nationallicence DSB : Double-strand breaks nationallicence ALS : Alkali-labile sites nationallicence OP : Organophosphate nationallicence CYPs : Cytochrome P450s nationallicence O2 : Oxygen nationallicence $${\text{O}}_{ 2}^{\cdot - }$$ O 2 · - : Superoxide anion nationallicence $$^{ \cdot } {\text{OH}}$$ · OH : Hydroxyl radicals nationallicence SOD : Superoxide dismutase nationallicence GSH-Px : Glutathione peroxidase nationallicence Endo III : Endonuclease III nationallicence FPG : Formamidopyrimidine DNA glycosylase nationallicence FISH : Fluorescence in situ hybridization nationallicence MDA : Malondialdehyde nationallicence PBS : Phosphate-buffered saline nationallicence OTM : Olive tail moment nationallicence SSC : Saline sodium citrate nationallicence Hb : Hemoglobin nationallicence AP sites : Apurinic or apyrimidinic sites nationallicence H2O2 : Hydrogen peroxide nationallicence Zhao Fei Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Wang Bai Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Zhang Xiaona Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Tian Hua Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Wang Wei Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Ru Shaoguo Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 613-624 0920-1742 41:3<613 2015 41 10695 https://doi.org/10.1007/s10695-015-0032-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0032-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Fei Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 700 1- Wang Bai Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiaona Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 700 1- Tian Hua Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wei Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 700 1- Ru Shaoguo Marine Life Science College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, Shandong Province, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 613-624 0920-1742 41:3<613 2015 41 10695