caa a22 4500 606229914 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-014-9992-x doi (NATIONALLICENCE)springer-10.1007/s10695-014-9992-x Molecular cloning and characterization of gonadotropin subunits (GTHα, FSHβ and LHβ) and their regulation by hCG and GnRHa in Japanese sea bass ( Lateolabrax japonicas ) in vivo [Elektronische Daten] [Mei Chi, Meng Ni, Ji Li, Feng He, Kun Qian, Pei Zhang, Sen Chai, Hai Wen] In this study, three cDNA sequences encoding common glycoprotein α subunit (GTHα), follicle-stimulating hormone β subunit (FSHβ) and luteinizing hormone β subunit (LHβ) were isolated from Japanese sea bass (Lateolabrax japonicas). Comparison of the deduced amino acid sequences with other gonadotropic hormones (GTHs) indicated that their cysteine residues and potential N-linked glycosylation sites were highly conserved, and high homology with those of other perciformes was showed in phylogenetic analysis. GTHs transcripts were present highly in the pituitary and brain and weakly in testis and other tissues. During testicular development, GTHs transcriptional levels in pituitary and brain (expect FSHβ subunit in brain) were significantly increased at spermiation period, stage V. Subsequently, the effects of hCG and GnRHa on the mRNA levels of GTHs subunits were examined. In brain, both hormones were detected to improve the expression of GTHα subunit mRNA. In pituitary, three GTHs subunits increased parallelly and abruptly in two hormone treatment groups. In testis, hCG was suggested to improve three GTHs subunits expression in Japanese sea bass for the first time. These results suggest that both gonadotropins are probably involved in the control of Japanese sea bass spermatogenesis and provide a framework for better understanding of the mechanisms of hormone-mediated reproduction control in Japanese sea bass and other teleosts. Springer Science+Business Media Dordrecht, 2015 Japanese sea bass nationallicence Gonadotropin subunits nationallicence Testis nationallicence hCG and GnRHa administration nationallicence GTH : Gonadotropic hormone nationallicence FSH : Follicle-stimulating hormone nationallicence LH : Luteinizing hormone nationallicence hCG : Human chorionic gonadotropin nationallicence GnRHa : Gonadotropin-releasing hormone analogue nationallicence M-MLV : Moloney murine leukemia virus nationallicence PS : Physiological saline nationallicence PGC : Primordial germ cell nationallicence PMSG : Pregnant mare serum gonadotropin nationallicence CDS : Coding sequence nationallicence NCBI : National Center for Biotechnical Information nationallicence Chi Mei Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Ni Meng Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Li Ji Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut He Feng Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Qian Kun Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Zhang Pei Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Chai Sen Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Wen Hai Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 587-601 0920-1742 41:3<587 2015 41 10695 https://doi.org/10.1007/s10695-014-9992-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9992-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chi Mei Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Ni Meng Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Li Ji Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- He Feng Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Qian Kun Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Pei Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Chai Sen Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 700 1- Wen Hai Fisheries College, Ocean University of China, 5 Yushan Road, 266003, Qingdao, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 587-601 0920-1742 41:3<587 2015 41 10695