caa a22 4500 606229930 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0043-z doi (NATIONALLICENCE)springer-10.1007/s10695-015-0043-z Zebrafish vitamin K epoxide reductases: expression in vivo, along extracellular matrix mineralization and under phylloquinone and warfarin in vitro exposure [Elektronische Daten] [Ignacio Fernández, Parameswaran Vijayakumar, Carlos Marques, M. Cancela, Paulo Gavaia, Vincent Laizé] Vitamin K (VK) acts as a cofactor driving the biological activation of VK-dependent proteins and conferring calcium-binding properties to them. As a result, VK is converted into VK epoxide, which must be recycled by VK epoxide reductases (Vkors) before it can be reused. Although VK has been shown to play a central role in fish development, particularly during skeletogenesis, pathways underlying VK actions are poorly understood, while good and reliable molecular markers for VK cycle/homeostasis are still lacking in fish. In the present work, expression of 2 zebrafish vkor genes was characterized along larval development and in adult tissues through qPCR analysis. Zebrafish cell line ZFB1 was used to evaluate in vitro regulation of vkors and other VK cycle-related genes during mineralization and upon 24h exposure to 0.16 and 0.8µM phylloquinone (VK1), 0.032µM warfarin, or a combination of both molecules. Results showed that zebrafish vkors are differentially expressed during larval development, in adult tissues, and during cell differentiation/mineralization processes. Further, several VK cycle intermediates were differentially expressed in ZFB1 cells exposed to VK1 and/or warfarin. Present work provides data identifying different developmental stages and adult tissues where VK recycling is probably highly required, and shows how genes involved in VK cycle respond to VK nutritional status in skeletal cells. Expression of vkor genes can represent a reliable indicator to infer VK nutritional status in fish, while ZFB1 cells could represent a suitable in vitro tool to get insights into the mechanisms underlying VK action on fish bone. Springer Science+Business Media Dordrecht, 2015 Vitamin K epoxide reductase nationallicence In vitro cell systems nationallicence Gene expression nationallicence Warfarin nationallicence Vitamin K nationallicence Danio rerio nationallicence Fernández Ignacio Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Vijayakumar Parameswaran Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Marques Carlos Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Cancela M. Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Gavaia Paulo Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Laizé Vincent Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 745-759 0920-1742 41:3<745 2015 41 10695 https://doi.org/10.1007/s10695-015-0043-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0043-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernández Ignacio Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Vijayakumar Parameswaran Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Marques Carlos Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Cancela M. Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Gavaia Paulo Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 700 1- Laizé Vincent Centre of Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 745-759 0920-1742 41:3<745 2015 41 10695