caa a22 4500 606229949 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0040-2 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0040-2 Roles of selenoprotein antioxidant protection in zebrafish, Danio rerio , subjected to dietary oxidative stress [Elektronische Daten] [M. Betancor, P. Almaida-Pagán, M. Sprague, A. Hernández, D. Tocher] In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid (DHA), are essential nutrients for fish although high dietary levels may lead to increased oxidative stress due to the high degree of unsaturation. The present study investigated the effects of Se supplementation on zebrafish, Danio rerio, oxidative status together with selenoprotein expression profiles when subjected to a high-DHA diet. Fish were fed for 8weeks with one of the four experimental diets, containing high or low DHA in combination with or without organic Se (7mg/kg). Fish performance, Se content, fatty acid composition and TBARS of zebrafish were determined, as well as gene expression of selected selenoproteins in liver and muscle. The Se levels in whole fish reflected dietary content. High dietary DHA increased oxidative stress as indicated by reduced growth and high TBARS content, although Se supplementation reduced oxidation. The expression patterns of selenoproteins varied between liver and muscle with only deiodinase type II displaying a transcriptional response when high dietary Se was supplied. High dietary DHA decreased selenoprotein W expression in muscle and sps2 expression in liver regardless of the dietary Se content. These data suggest that oxidative stress protection associated with a high dietary intake of Se may not be solely mediated by transcriptional changes in teleost selenoprotein expression. Springer Science+Business Media Dordrecht, 2015 Zebrafish nationallicence Selenium nationallicence Docosahexaenoic acid nationallicence Oxidative stress nationallicence Selenoproteins nationallicence BACT : Beta-actin nationallicence B2M : Beta-2-microglobulin nationallicence DIO2 : Deiodinase type II nationallicence DHA : Docosahexaenoic acid nationallicence FAME : Fatty acid methyl esters nationallicence GPX : Glutathione peroxidase nationallicence LC-PUFA : Long-chain polyunsaturated fatty acid nationallicence NTC : No-template control nationallicence PIn : Peroxidation index nationallicence qPCR : Quantitative PCR nationallicence RO : Rapeseed oil nationallicence ROS : Reactive oxygen species nationallicence SGR : Specific growth rate nationallicence SEM : Standard error of the mean nationallicence SECP43 : tRNA selenocysteine 1-associated protein 1b nationallicence SEP15 : 15kDa Selenoprotein nationallicence SEPP1a : Selenoprotein P1 nationallicence SEPW : Selenoprotein W nationallicence SPS2 : Selenophosphate synthetase 2 nationallicence TBARS : Thiobarbituric acid reactive substances nationallicence TCA : Trichloroacetic acid nationallicence TXNRD1 : Thioredoxin reductase 1 nationallicence Betancor M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut Sprague M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut Hernández A. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 705-720 0920-1742 41:3<705 2015 41 10695 https://doi.org/10.1007/s10695-015-0040-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0040-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Betancor M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut NATIONALLICENCE 700 1- Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut NATIONALLICENCE 700 1- Sprague M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut NATIONALLICENCE 700 1- Hernández A. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut NATIONALLICENCE 700 1- Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, UK aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 705-720 0920-1742 41:3<705 2015 41 10695