caa a22 4500 606229957 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0049-6 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0049-6 Effects of dietary glucose and dextrin on activity and gene expression of glucokinase and fructose-1,6-bisphosphatase in liver of turbot Scophthalmus maximus [Elektronische Daten] [Qin Nie, Huijun Miao, Shuyan Miao, Huihui Zhou, Yanjiao Zhang, Wenbing Zhang, Kangsen Mai] Glucokinase (GK) and fructose-1,6-bisphosphatase (FBPase) play crucial role in glucose metabolism. In the present study, the cDNA encoding GK and FBPase was cloned from the liver of turbot Scophthalmus maximus by rapid amplification of cDNA end technique. Effects of dietary glucose and dextrin on the activities and gene expressions of these two enzymes were also studied. Results showed that the full length of GK cDNA was 2226bp, consisting of an open reading frame (ORF) of 1434bp. The full-length cDNA coding FBPase was 1314bp with a 1014bp ORF encoding 337 amino acids. Analyses of gene expression of GK and FBPase were conducted in gill, liver, the whole intestine, the whole kidney, heart, the dorsal white muscle and brain. The highest expression of GK was found in liver, followed by muscle. The expression of FBPase was found higher in liver than heart and gill. Both hepatic GK activity and mRNA expression were highly induced in turbot after being fed with dietary carbohydrates (p<0.05). However, the GK activity and mRNA expression in the group with dietary glucose did not significantly differ from those in the group with dietary dextrin (p>0.05). Compared with the control group, there were no significant differences in FBPase activity and mRNA expression in the glucose as well as dextrin group (p>0.05). The increased hepatic GK activity and gene expression indicated that the first step of glycolysis was activated in turbot by dietary carbohydrates. Springer Science+Business Media Dordrecht, 2015 Turbot nationallicence Glucokinase nationallicence Fructose-1,6-bisphosphatase nationallicence Carbohydrate nationallicence Nutrition nationallicence Nie Qin The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Miao Huijun The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Miao Shuyan The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Zhou Huihui The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Zhang Yanjiao The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Zhang Wenbing The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Mai Kangsen The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 819-832 0920-1742 41:3<819 2015 41 10695 https://doi.org/10.1007/s10695-015-0049-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0049-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nie Qin The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Miao Huijun The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Miao Shuyan The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Huihui The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yanjiao The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wenbing The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Mai Kangsen The Key Laboratory of Aquaculture Nutrition and Feeds, Ministry of Agriculture, The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 819-832 0920-1742 41:3<819 2015 41 10695