caa a22 4500 606229965 CHVBK 20210128101211.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0048-7 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0048-7 Raymond James School of Life Sciences, University of Nevada Las Vegas, 89154, Las Vegas, NV, USA aut Two potential fish glycerol-3-phosphate phosphatases [Elektronische Daten] [James Raymond] Winter-acclimated rainbow smelt (Osmerus mordax Mitchill) produce high levels of glycerol as an antifreeze. A common pathway to glycerol involves the enzyme glycerol-3-phosphate phosphatase (GPP), but no GPP has yet been identified in fish or any other animal. Here, two phosphatases assembled from existing EST libraries (from winter-acclimated smelt and cold-acclimated smelt hepatocytes) were found to resemble a glycerol-associated phosphatase from a glycerol-producing alga, Dunaliella salina, and a recently discovered GPP from a bacterium, Mycobacterium tuberculosis. Recombinant proteins were generated and were found to have GPP activity on the order of a few μMol Pi/mg enzyme/min. The two enzymes have acidic pH optima (~5.5) similar to that previously determined for GPP activity in liver tissue, with about 1/3 of their peak activities at neutral pH. The two enzymes appear to account for the GPP activity of smelt liver, but due to their reduced activities at neutral pH, their contributions to glycerol production in vivo remain unclear. Similar enzymes may be active in a glycerol-producing insect, Dendroctonus ponderosae. Springer Science+Business Media Dordrecht, 2015 Phosphoserine phosphatase nationallicence serB nationallicence Osmerus mordax nationallicence Dunaliella salina nationallicence Mycobacterium tuberculosis nationallicence Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 811-818 0920-1742 41:3<811 2015 41 10695 https://doi.org/10.1007/s10695-015-0048-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0048-7 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Raymond James School of Life Sciences, University of Nevada Las Vegas, 89154, Las Vegas, NV, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 811-818 0920-1742 41:3<811 2015 41 10695