caa a22 4500 60622999X CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0036-y doi (NATIONALLICENCE)springer-10.1007/s10695-015-0036-y Establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish ( Misgurnus anguillicaudatus ) [Elektronische Daten] [Xia Li, Chen Ma, Yan-Jie Qin, Ya-Juan Li, Di Wu, Li-Wen Bai, Ai-Jun Pei] Continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, Misgurnus anguillicaudatus, were established and characterized. The cell lines, designated DIMF, TRMF, and TEMF, respectively, were subcultured more than 80 times since initiation in October 2012 and were preserved at the China Center for Type Culture Collection as sample numbers C2013109, C2013110, C2013111, respectively. The cell lines consist predominantly of fibroblast-like cells. At the 50th passage, the population doubling times were 48.43h (DIMF), 36.01h (TRMF), and 41.45h (TEMF). Cell survival rate of these three kinds of cells was 80.88±1.38, 84.48±1.13, and 81.57±1.28%, respectively, when recovered after storage in liquid nitrogen for 60days at the 40th passage. The chromosome numbers measured from 100 metaphase plates at the 50th passage were 2n=50 (68%), 3n=75 (59%), and 4n=100 (54%) for DIMF, TRMF, and TEMF cells, respectively. At the 60th passage, the chromosome numbers for DIMF and TRMF cells were still 50 (52%) and 75 (70%), but the chromosome number for TEMF cells ranged from 88 to 100; a chromosome number of 96 accounted for 26% of the cells, and the karyotype analysis showed 4n=96, 16m+8sm+72t, NF=120; thus, compared with cells at the 50th passage, a group of metacentric chromosomes was missing. Microsatellite marker analysis was conducted using DIMF, TRMF, and TEMF cells and muscle tissue of oriental weatherfish, which confirmed that the three cell lines established in this study were from oriental weatherfish. The cell lines were exposed to two fish viruses to determine their susceptibility to infection; they were susceptible to spring viremia of carp virus but not to piscine nodavirus. Establishment of fin cell lines from different ploidy oriental weatherfish increases the existing number of fish cell lines available for research, and it provides a model for investigating the mechanisms of growth and genetics in polyploid fish. Springer Science+Business Media Dordrecht, 2015 Misgurnus anguillicaudatus nationallicence Cell line nationallicence Polyploidy nationallicence Karyotype nationallicence Virus susceptibility nationallicence Li Xia Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Ma Chen Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Qin Yan-Jie Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Li Ya-Juan Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Wu Di Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Bai Li-Wen Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Pei Ai-Jun Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 661-672 0920-1742 41:3<661 2015 41 10695 https://doi.org/10.1007/s10695-015-0036-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0036-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xia Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Ma Chen Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Qin Yan-Jie Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Li Ya-Juan Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Wu Di Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Bai Li-Wen Key Laboratory of Mariculture, Agriculture Ministry, PRC, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 700 1- Pei Ai-Jun Key Laboratory of Marine Bio-resource Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Heishijiao Street 52, 116023, Dalian, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 661-672 0920-1742 41:3<661 2015 41 10695