caa a22 4500 606230033 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0033-1 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0033-1 Neurotoxic effects of nickel chloride in the rainbow trout brain: Assessment of c-Fos activity, antioxidant responses, acetylcholinesterase activity, and histopathological changes [Elektronische Daten] [Ahmet Topal, Muhammed Atamanalp, Ertan Oruç, Mesut Halıcı, Melda Şişecioğlu, Hüseyin Erol, Arzu Gergit, Bahar Yılmaz] The aim of this study was to determine the biochemical, immunohistochemical, and histopathological effects of nickel chloride (Ni) in the rainbow trout brain. Fish were exposed to Ni concentrations (1mg/L and 2mg/L) for 21days. At the end of the experimental period, brain tissues were taken from all fish for c-Fos activity and histopathological examination and determination of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT) enzyme activities, lipid peroxidation (LPO), and glutathione (GSH) levels. Our results showed that Ni treatment caused a significant increase in the brain SOD activity and in LPO and GSH levels (p<0.05), but it significantly decreased AChE and CAT enzyme activities (p<0.05). Strong induction in c-Fos was observed in some cerebral and cerebellar regions of fish exposed to Ni concentrations when compared with the control group. However, c-Fos activity was decreased in necrotic Purkinje cells. Brain tissues were characterized by demyelination and necrotic changes. These results suggested that Ni treatment causes oxidative stress, changes in c-Fos activity, and histopathological damage in the fish brain. Springer Science+Business Media Dordrecht, 2015 Nickel nationallicence Fish nationallicence Brain nationallicence c-Fos nationallicence Toxicity nationallicence Oxidative stress nationallicence Topal Ahmet Department of Basic Sciences, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut Atamanalp Muhammed Department of Aquaculture, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut Oruç Ertan Department of Pathology, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut Halıcı Mesut Department of Biochemistry, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut Şişecioğlu Melda Department of Molecular Biology and Genetics, Faculty of Sciences, Ataturk University, 25240, Erzurum, Turkey aut Erol Hüseyin Department of Biochemistry, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut Gergit Arzu Department of Basic Sciences, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut Yılmaz Bahar Department of Molecular Biology and Genetics, Faculty of Sciences, Ataturk University, 25240, Erzurum, Turkey aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 625-634 0920-1742 41:3<625 2015 41 10695 https://doi.org/10.1007/s10695-015-0033-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0033-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Topal Ahmet Department of Basic Sciences, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Atamanalp Muhammed Department of Aquaculture, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Oruç Ertan Department of Pathology, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Halıcı Mesut Department of Biochemistry, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Şişecioğlu Melda Department of Molecular Biology and Genetics, Faculty of Sciences, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Erol Hüseyin Department of Biochemistry, Faculty of Veterinary, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Gergit Arzu Department of Basic Sciences, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 700 1- Yılmaz Bahar Department of Molecular Biology and Genetics, Faculty of Sciences, Ataturk University, 25240, Erzurum, Turkey aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 625-634 0920-1742 41:3<625 2015 41 10695