caa a22 4500 606230041 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0038-9 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0038-9 Spatial learning associated with stimulus response in goldfish Carassius auratus: relationship to activation of CREB signalling [Elektronische Daten] [Koilmani Rajan, Subramanian Thangaleela, Chellam Balasundaram] Earlier, we reported spatial learning ability in goldfish (Carassius auratus) by using spatial paradigm with food reward. Therefore, we hypothesized that goldfish may use associated cue to integrate "where” and "what” for spatial memory. To test our hypothesis, we first trained goldfish to learn to cross the gate1, which is associated with spatial task. Subsequently, they were trained to learn to enter the task chamber and to identify the food reward chamber associated with visual cue (red/green light). Red and green lights were positioned randomly for each trial but always the food reward was kept in green chamber. In addition, to elucidate the role of the signalling cascade in spatial memory associated with visual cue, nicotinamide (NAM, 1000mg/kg, i.p), a NAD+ precursor, was used to inhibit the Sirtuin 1 (SIRT1) cyclic AMP response element binding protein (CREB) pathway. Fishes were trained for 5days in a maze after treating with either vehicle (VEH, DD H2O) or NAM, and then, they were individually tested for memory. We found that VEH-treated fish learned and recalled the task successfully by showing less latency and making more correct choices than NAM-treated group. Subsequent analysis showed that NAM treatment significantly down-regulated the phosphorylation of extracellular signal-regulated kinase (ERK1/2), CREB, expression of SirT1 and brain-derived neurotrophic factor (Bdnf) in telencephalon. Taken together, our results provide behavioural evidence of spatial memory associated with visual cue in C. auratus, which could be regulated by ERK1/2-CREB-SirT1-Bdnf pathway. Springer Science+Business Media Dordrecht, 2015 Spatial memory nationallicence Visual cue nationallicence Carassius auratus nationallicence Nicotinamide nationallicence ERK1/2 nationallicence CREB nationallicence SirT1 nationallicence Bdnf nationallicence Rajan Koilmani Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut Thangaleela Subramanian Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut Balasundaram Chellam Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 685-694 0920-1742 41:3<685 2015 41 10695 https://doi.org/10.1007/s10695-015-0038-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0038-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rajan Koilmani Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 700 1- Thangaleela Subramanian Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 700 1- Balasundaram Chellam Department of Animal Science, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 685-694 0920-1742 41:3<685 2015 41 10695