caa a22 4500 606230076 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10695-015-0045-x doi (NATIONALLICENCE)springer-10.1007/s10695-015-0045-x Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish ( Danio rerio ) [Elektronische Daten] [Juan Tian, Gen He, Kangsen Mai, Chengdong Liu] The goal of this study was to systematically evaluate the molecular activities of endocrine-, amino acid and peptide transporters-, and metabolic enzyme-related genes in 35-day-old mixed-sex zebrafish (Danio rerio) after feeding . Zebrafish with initial body weights ranging from 9 to 11mg were fasted for 384h in a controlled indoor environment. Fish were sampled at 0, 3, 6, 12, 24, 48, 96, 192, and 384h after fed. Overall, the present study results show that the regulatory mechanism that insulin-like growth factor I negative feedback regulated growth hormone is conserved in zebrafish, as it is in mammals, but that regulation of growth hormone receptors is highly intricate. Leptin and cholecystokinin are time-dependent negative feedback signals, and neuropeptide Y may be an important positive neuropeptide for food intake in zebrafish. The amino acid/carnitine transporters B0,+ (ATB0,+) and broad neutral (0) amino acid transporter 1(B0AT1) mRNA levels measured in our study suggest that protein may be utilized during 24-96h of fasting in zebrafish. Glutamine synthetase mRNA levels were downregulated, and glutamate dehydrogenase, alanine aminotransferase, aspartate transaminase, and trypsin mRNA levels were upregulated after longtime fasting in this study. The mRNA expression levels of fatty acid synthetase decreased significantly (P < 0.05), whereas those of lipoprotein lipase rapidly increased after 96h of fasting. Fasting activated the expression of glucose synthesis genes when fasting for short periods of time; when fasting is prolonged, the mRNA levels of glucose breakdown enzymes and pentose phosphate shunt genes decreased. Springer Science+Business Media Dordrecht, 2015 Postprandial starvation nationallicence Transporters nationallicence Metabolic enzymes nationallicence Zebrafish nationallicence Tian Juan Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut He Gen Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut Mai Kangsen Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut Liu Chengdong Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 773-787 0920-1742 41:3<773 2015 41 10695 https://doi.org/10.1007/s10695-015-0045-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0045-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tian Juan Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- He Gen Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Mai Kangsen Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Liu Chengdong Key Laboratory of Aquaculture Nutrition (Ministry of Agriculture), Ocean University of China, No. 5 Yushan Rd., 266003, Qingdao, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/3(2015-06-01), 773-787 0920-1742 41:3<773 2015 41 10695