caa a22 4500 606230106 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0067-4 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0067-4 Sulphated glycosaminoglycans and proteoglycans in the developing vertebral column of juvenile Atlantic salmon ( Salmo salar ) [Elektronische Daten] [Kirsten Hannesson, Elisabeth Ytteborg, Harald Takle, Grethe Enersen, Grete Bæverfjord, Mona Pedersen] In the present study, the distribution of sulphated glycosaminoglycans (GAGs) in the developing vertebral column of Atlantic salmon (Salmo salar) at 700, 900, 1100 and 1400d° was examined by light microscopy. The mineralization pattern was outlined by Alizarin red S and soft structures by Alcian blue. The temporal and spatial distribution patterns of different types of GAGs: chondroitin-4-sulphate/dermatan sulphate, chondroitin-6-sulphate, chondroitin-0-sulphate and keratan sulphate were addressed by immunohistochemistry using monoclonal antibodies against the different GAGs. The specific pattern obtained with the different antibodies suggests a unique role of the different GAG types in pattern formation and mineralization. In addition, the distribution of the different GAG types in normal and malformed vertebral columns from 15g salmon was compared. A changed expression pattern of GAGs was found in the malformed vertebrae, indicating the involvement of these molecules during the pathogenesis. The molecular size of proteoglycans (PGs) in the vertebrae carrying GAGs was analysed with western blotting, and mRNA transcription of the PGs aggrecan, decorin, biglycan, fibromodulin and lumican by real-time qPCR. Our study reveals the importance of GAGs in development of vertebral column also in Atlantic salmon and indicates that a more comprehensive approach is necessary to completely understand the processes involved. The Author(s), 2015 Glycosaminoglycans nationallicence Proteoglycans nationallicence Vertebral development nationallicence Salmon nationallicence Hannesson Kirsten Nofima AS, 1430, Ås, Norway aut Ytteborg Elisabeth Nofima AS, 1430, Ås, Norway aut Takle Harald Nofima AS, 1430, Ås, Norway aut Enersen Grethe Nofima AS, 1430, Ås, Norway aut Bæverfjord Grete Nofima AS, 6600, Sunndalsøra, Norway aut Pedersen Mona Nofima AS, 1430, Ås, Norway aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1029-1051 0920-1742 41:4<1029 2015 41 10695 https://doi.org/10.1007/s10695-015-0067-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0067-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hannesson Kirsten Nofima AS, 1430, Ås, Norway aut NATIONALLICENCE 700 1- Ytteborg Elisabeth Nofima AS, 1430, Ås, Norway aut NATIONALLICENCE 700 1- Takle Harald Nofima AS, 1430, Ås, Norway aut NATIONALLICENCE 700 1- Enersen Grethe Nofima AS, 1430, Ås, Norway aut NATIONALLICENCE 700 1- Bæverfjord Grete Nofima AS, 6600, Sunndalsøra, Norway aut NATIONALLICENCE 700 1- Pedersen Mona Nofima AS, 1430, Ås, Norway aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1029-1051 0920-1742 41:4<1029 2015 41 10695