caa a22 4500 606230114 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0051-z doi (NATIONALLICENCE)springer-10.1007/s10695-015-0051-z Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus [Elektronische Daten] [M. Di Yorio, T. Delgadin, D. Pérez Sirkin, P. Vissio] Neuropeptide Y (NPY) is considered the most potent orexigenic peptide, increasing before meal time and during fasting. In teleost, most studies on NPY action upon growth hormone (GH) and luteinizing hormone (LH) were conducted in females or group of animals without sex discrimination. The aim of this study was to evaluate whether NPY modulates the expression and release of GH and gonadotropins in both sexes of Cichlasoma dimerus. By double-label immunofluorescence, we first determined the association between NPY fibers and pituitary cells. In addition, we performed in vitro studies to evaluate the effect of NPY on GH and gonadotropins expression by real-time PCR, and release by Western blot, in males and females separately. Contacts between NPY fibers and GH and follicle-stimulating hormone (FSH)-producing cells were detected, indicating possible functional relationships. We observed an increase in GH release in the culture medium at 2nM for males (p=0.043) and 20nM for females (p=0.028). Pituitary FSH release was stimulated at 20 nM (p=0.026) and 200nM (p=0.033) for males and females, respectively. Finally, NPY only increased β-LH mRNA expression at 20nM in females (p=0.028) and its release at 2nM (p=0.049) and 200nM for males (p=0.005) and 200nM for females (p=0.018). In conclusion, NPY acts as a GH-, LH- and FSH-releasing factor, in a dose- and sex-dependent way. Springer Science+Business Media Dordrecht, 2015 NPY nationallicence GH nationallicence FSH nationallicence LH nationallicence Cichlids nationallicence Di Yorio M. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut Delgadin T. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut Pérez Sirkin D. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut Vissio P. IBBEA-CONICET-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 843-852 0920-1742 41:4<843 2015 41 10695 https://doi.org/10.1007/s10695-015-0051-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0051-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Yorio M. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Delgadin T. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Pérez Sirkin D. Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Vissio P. IBBEA-CONICET-UBA, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 843-852 0920-1742 41:4<843 2015 41 10695