caa a22 4500 606230157 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0059-4 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0059-4 Changes in the physiological parameters, fatty acid metabolism, and SCD activity and expression in juvenile GIFT tilapia ( Oreochromis niloticus ) reared at three different temperatures [Elektronische Daten] [X. Ma, J. Qiang, J. He, N. Gabriel, P. Xu] We evaluated the effects of rearing temperature on the composition of fatty acids and stearoyl-CoA desaturase (SCD) activity and gene expression in GIFT (genetically improved farmed tilapia) tilapia. Three triplicate groups of fish were reared for 40days at 22, 28, or 34°C. At the end of the trial, the final body weight of juveniles reared at 28°C was higher than that of fish reared at 22 or 34°C. Feed intake, feed efficiency, and the protein efficiency ratio were also higher at 28°C. The fatty acid composition of muscle tissue differed significantly (P<0.05) among the treatment groups. The content of SFA decreased with decreasing temperature, whereas the UFA content increased. We observed high levels of PUFA, particularly n-3 PUFAs, in fish reared at the lower temperature. Rearing at low temperature significantly (P<0.05) increased the expression and activity of the SCD gene. Increased SCD activity and gene expression can increase the biosynthesis of MUFAs in GIFT tilapia muscle. Additionally, cold acclimation can decrease the content of TC and TG in GIFT tilapia, which can help increase cold tolerance. Springer Science+Business Media Dordrecht, 2015 Oreochromis niloticus nationallicence Temperature nationallicence Fatty acid nationallicence Stearoyl-CoA desaturase nationallicence Blood parameters nationallicence Ma X. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut Qiang J. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut He J. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081, Wuxi, Jiangsu, China aut Gabriel N. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut Xu P. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 937-950 0920-1742 41:4<937 2015 41 10695 https://doi.org/10.1007/s10695-015-0059-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0059-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma X. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut NATIONALLICENCE 700 1- Qiang J. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut NATIONALLICENCE 700 1- He J. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, 214081, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Gabriel N. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut NATIONALLICENCE 700 1- Xu P. Fisheries College, Nanjing Agriculture University, 214081, Wuxi, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 937-950 0920-1742 41:4<937 2015 41 10695