caa a22 4500 606230165 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0062-9 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0062-9 Physiological management of dietary deficiency in n-3 fatty acids by spawning Gulf killifish ( Fundulus grandis ) [Elektronische Daten] [Joshua Patterson, Christopher Green] Lipid dynamics of spawning fish are critical to the production of viable embryos and larvae. The present study utilized manipulation of dietary fatty acid (FA) profiles to examine the ability of spawning Gulf killifish (Fundulus grandis) to mobilize critical lipid components from somatic reserves or synthesize long-chain polyunsaturated FAs (LC-PUFAs) de novo from shorter-chain C18 precursors. An egg and multi-tissue evaluation of changes in FA concentrations across time after fish were switched from LC-PUFA-rich to LC-PUFA-deficient experimental diets was employed. The two experimental diets contained lipid sources which differed drastically in n-3 C18 FA content but had similar levels of n-6 C18 FAs. Discrete effects of dietary n-3 FAs can be analyzed because n-3 and n-6 represent distinct metabolic families which cannot be exchanged in vivo. Results indicate that a combination of mobilization and de novo synthesis is likely utilized to maintain physiologically required FA levels in critical tissues and embryos. Mobilization was supported by decreases in LC-PUFAs in somatic tissues and decreases in intraperitoneal fat content and liver mass. Evidence for biosynthesis was provided by a higher level of n-3 LC-PUFAs in the liver and ova of fish fed diets containing n-3 C18 precursors versus those fed diets with low levels of precursor FAs. The characteristic physiological plasticity of Gulf killifish is exemplified in the nutritional domain by its management of dietary FA deficiency. Springer Science+Business Media Dordrecht, 2015 Fatty acids nationallicence Fundulus nationallicence Gulf killifish nationallicence Lipid mobilization nationallicence Reproduction nationallicence Patterson Joshua Aquaculture Research Station, Louisiana State University Agricultural Center, 70820, Baton Rouge, LA, USA aut Green Christopher Aquaculture Research Station, Louisiana State University Agricultural Center, 70820, Baton Rouge, LA, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 971-979 0920-1742 41:4<971 2015 41 10695 https://doi.org/10.1007/s10695-015-0062-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0062-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Patterson Joshua Aquaculture Research Station, Louisiana State University Agricultural Center, 70820, Baton Rouge, LA, USA aut NATIONALLICENCE 700 1- Green Christopher Aquaculture Research Station, Louisiana State University Agricultural Center, 70820, Baton Rouge, LA, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 971-979 0920-1742 41:4<971 2015 41 10695