caa a22 4500 606230173 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0066-5 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0066-5 Physiological response of alligator gar juveniles ( Atractosteus spatula ) exposed to sub-lethal doses of pollutants [Elektronische Daten] [Carlos González, Julio Cruz, Roberto Alfaro] Alligator gar populations have declined because of overfishing, habitat loss and pollution. Over time, the exposure to different pollutants have affected these fishes as a consequence of their high trophic level, bottom-dwelling habits and long life span. In order to evaluate the physiological effects of pollutants on alligator gar, juveniles (6, 12 and 24months) were exposed to sub-lethal doses of diazinon, β-naphthoflavone (BNF) and 17 β-estradiol (E2) by intraperitoneal injection. After 2days of exposure, liver samples were taken to determine the activities of acetylcholinesterase, butyrylcholinesterase and carboxylesterase; alkaline and acid phosphatases (ALP and ACP); ethoxyresorufin o-deethylase (EROD); glutathione s-transferase (GST); superoxide dismutase (SOD), and vitellogenin (VTG) concentration. Two additional bioassays consisting on the exposure of compounds through water or food were performed and after 4 and 28days, respectively, biomarkers were determined. All esterases were inhibited in organisms exposed to diazinon as well as in 6-months gar exposed to E2 and BNF. In contrast, ALP activity increased in gar exposed to diazinon and E2, while ACP activity did not show any variations. No EROD activity was registered after exposure to the different pollutants, despite being one of the most sensitive and common detoxification biomarkers used for fishes. GST activity reduction was detected when gar were exposed to E2 and BNF, while SOD activity increased after exposure to diazinon and E2. Finally, VTG levels were higher in animals exposed to E2 compared to other treatments. Overall, these results suggest that alligator gar juveniles have a low biotransformation metabolism and show that they are especially sensitive to those pollutants affecting the nervous system. Springer Science+Business Media Dordrecht, 2015 Alligator gar nationallicence Biotransformation enzymes nationallicence Cholinesterases nationallicence Vitellogenin nationallicence EDCs nationallicence González Carlos Laboratorio de Ecofisiología, Departamento de Ecología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Apartado Postal F-96, C.P. 66450, San Nicolás de los Garza, Nuevo León, Mexico aut Cruz Julio Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León (UANL), C.P. 66450, Escobedo, Nuevo León, Mexico aut Alfaro Roberto Laboratorio de Ecofisiología, Departamento de Ecología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Apartado Postal F-96, C.P. 66450, San Nicolás de los Garza, Nuevo León, Mexico aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1015-1027 0920-1742 41:4<1015 2015 41 10695 https://doi.org/10.1007/s10695-015-0066-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0066-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- González Carlos Laboratorio de Ecofisiología, Departamento de Ecología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Apartado Postal F-96, C.P. 66450, San Nicolás de los Garza, Nuevo León, Mexico aut NATIONALLICENCE 700 1- Cruz Julio Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León (UANL), C.P. 66450, Escobedo, Nuevo León, Mexico aut NATIONALLICENCE 700 1- Alfaro Roberto Laboratorio de Ecofisiología, Departamento de Ecología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Apartado Postal F-96, C.P. 66450, San Nicolás de los Garza, Nuevo León, Mexico aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1015-1027 0920-1742 41:4<1015 2015 41 10695