caa a22 4500 606230181 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0057-6 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0057-6 Spermatogenesis and related plasma androgen and progestin level in wild male golden mahseer, Tor putitora (Hamilton, 1822), during the spawning season [Elektronische Daten] [Neetu Shahi, Sumanta Mallik, Jyoti Pande, Partha Das, Atul Singh] Testicular development and plasma levels of sex steroid [11-ketotestosterone (11-KT), testosterone (T) and 17,20β-dihydoxy-4-pregnen-3-one (17,20β-P)] were studied for the first time in wild golden mahseer, Tor putitora. Testicular development was investigated by macroscopic observation and histology of the gonads, whereas steroids were measured by enzyme-linked immunosorbent assay. Based on macroscopic observation and germ cell types present in gonad histology, the testes of T. putitora were divided into five developmental stages: immature [stage I; spermatogonia (SPG)], early spermatogenesis [stage II; SPG and spermatocytes (SPC)], late spermatogenesis [stage III; SPG, SPC, spermatids (SPD) and spermatozoa (SPZ)], spermiation (stage IV; SPZ) and post-spawning (stage V; SPG, SPD and SPZ). During the stage I of the testes, the lowest levels of plasma sex steroid and gonadosomatic index (I G) were recorded. The highest plasma level of T was 0.89±0.09ng/mL and 11-KT was 4.23±0.54ng/mL, which was during the stage III and IV, respectively. The peak in 11-KT was coincident with the peak in I G (1.65±0.12%). The lowest T and 11-KT levels were 0.25±0.02ng/mL and 0.47±0.09ng/mL, respectively, which was at stage I. Plasma levels of 17,20β-P increased significantly at stage III (1.04±0.06ng/mL) and stage IV testes (1.28±0.03ng/mL) and then declined in post-spawned fish. This indicates that 17,20β-P could also be a possible maturation-inducing steroid in this fish. The condition factor (K) significantly decreased during the testicular development and was lowest at spermiation stage (0.53±0.02%). The proportion of running male peaked concomitantly with the appearance of stage IV testes. Presence of germ cells of different developmental stages indicates that T. putitora male is a multiple spawner, and the information generated here is important for developing a captive breeding, culture and conservation programs for this endangered coldwater Himalayan fish species. Springer Science+Business Media Dordrecht, 2015 Tor putitora nationallicence Maturation-inducing steroid nationallicence Testicular development nationallicence Testosterone nationallicence 11-Ketotestosterone nationallicence 17,20β-Dihydoxy-4-pregnen-3-one nationallicence Shahi Neetu Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut Mallik Sumanta Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut Pande Jyoti Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut Das Partha Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut Singh Atul Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 909-920 0920-1742 41:4<909 2015 41 10695 https://doi.org/10.1007/s10695-015-0057-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0057-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shahi Neetu Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Mallik Sumanta Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Pande Jyoti Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Das Partha Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut NATIONALLICENCE 700 1- Singh Atul Directorate of Coldwater Fisheries Research, 263 136, Bhimtal, Nainital, Uttarakhand, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 909-920 0920-1742 41:4<909 2015 41 10695