caa a22 4500 606230203 CHVBK 20210128101212.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0065-6 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0065-6 Effects of increased temperature on metabolic activity and oxidative stress in the first life stages of marble trout ( Salmo marmoratus ) [Elektronische Daten] [Tatjana Simčič, Dušan Jesenšek, Anton Brancelj] Climate change may result in future alterations in thermal regime which could markedly affect the early developmental stages of cold water fish due to their expected high sensitivity to increasing temperature. In the present study, the effect of temperature increase of 2, 4 and 6°C on the oxygen consumption rate (R), the activity of respiratory electron transport system (ETS) and oxidative stress have been studied in four developmental stages of the marble trout (Salmo marmoratus)—eyed eggs, yolk-sac larvae and juveniles of 1 and 3months. Oxygen consumption rate and ETS activity increased with level of development and with temperature in all four stages. ETS/R ratios decreased during development and correlated with temperature in eyed eggs, larvae and juveniles of 1month, but not in juveniles of 3months. Low ETS/R ratios at higher temperatures indicate stress response in eyed eggs, the most temperature sensitive developmental stage. Catalase (CAT) and glutathione reductase (GR) activities increased during development, but responded differently to elevated temperature in the different developmental stages. Stress in eyed eggs, caused by higher temperatures, resulted in increased oxygen consumption rate and increased activities of CAT and GR. Larvae were sensitive to increased temperature only at the highest experimental temperature of 16°C. Increased temperature did not stress the metabolism of the juveniles, since they were able to compensate their metabolic activity. The earlier developmental stages of marble trout are thus more sensitive to temperature increase than juveniles and therefore more endangered by higher water temperatures. This is the first report connecting oxygen consumption, ETS activity and ETS/R ratio with the activities of antioxidant enzymes in relation to increased temperature in salmonids. Springer Science+Business Media Dordrecht, 2015 Salmo marmoratus nationallicence Thermal stress nationallicence Respiration nationallicence ETS activity nationallicence ETS/R ratio nationallicence Antioxidant enzymes nationallicence Simčič Tatjana National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut Jesenšek Dušan Tolmin Angling Association, Tolmin, Slovenia aut Brancelj Anton National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1005-1014 0920-1742 41:4<1005 2015 41 10695 https://doi.org/10.1007/s10695-015-0065-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0065-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Simčič Tatjana National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Jesenšek Dušan Tolmin Angling Association, Tolmin, Slovenia aut NATIONALLICENCE 700 1- Brancelj Anton National Institute of Biology, Večna pot 111, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 1005-1014 0920-1742 41:4<1005 2015 41 10695