caa a22 4500 606230211 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0053-x doi (NATIONALLICENCE)springer-10.1007/s10695-015-0053-x Ontogenetic development of adipose tissue in grass carp ( Ctenopharyngodon idellus ) [Elektronische Daten] [Pin Liu, Hong Ji, Chao Li, Jingjing Tian, Yifei Wang, Ping Yu] To investigate the adipose tissue development process during the early stages of grass carp (Ctenopharyngodon idellus) development, samples were collected from fertilized eggs to 30days post-fertilization (dpf) of fish. Paraffin and frozen sections were taken to observe the characteristics of adipocytes in vivo by different staining methods, including hematoxylin and eosin (H&E), Oil red O, and BODIPY. The expression of lipogenesis-related genes of the samples at different time points was detected by real-time qPCR. In addition, protein expression level of peroxisome proliferator-activated receptors γ (PPAR γ) was detected by immunohistochemistry. The results showed that the neutral lipid droplets accumulated first in the hepatocytes of 14-dpf fish larvae, and visceral adipocytes appeared around the hepatopancreas on 16dpf. As grass carp grew, the adipocytes increased in number and spread to other tissues. In 20-dpf fish larvae, the intestine was observed to be covered by adipose tissue. However, there was no significant change in the average size (30.40-40.01μm) of adipocytes during this period. Accordingly, the gene expression level of PPAR γ and CCAAT/enhancer-binding proteins α (C/EBP α) was significantly elevated after fertilization for 12days (p<0.05), but C/EBP α declined at 20dpf. Expression of lipoprotein lipase (LPL) increased from 2 to 16dpf and then declined. In addition, immunoreaction of PPAR γ was positive on hepatocytes after fertilization for 15days. These results implied that the early developmental stage of adipose tissue is caused by active recruitment of adipocytes as opposed to hypertrophy of the cell. In addition, our study indicated that lipogenesis-related genes might regulate the ongoing development of adipose tissue. Springer Science+Business Media Dordrecht, 2015 Grass carp nationallicence Post-embryonic development nationallicence Adipocytes nationallicence Visceral adipose tissue nationallicence Histology nationallicence BAT : Brown adipose tissue nationallicence WAT : White adipose tissue nationallicence CVD : Cardiovascular disease nationallicence PPAR γ : Peroxisome proliferator-activated receptors nationallicence LPL : Lipoprotein lipase nationallicence dpf : Days post-fertilization nationallicence H&E : Hematoxylin and eosin nationallicence PBS : Phosphate-buffered saline nationallicence APES : 3-Aminopropyl-triethoxysilane nationallicence TBS : Tris buffer solution nationallicence BSA : Bovine serum albumin nationallicence C/EBP α : CCAAT/enhancer-binding proteins α nationallicence FAS : Fatty acid synthase nationallicence Liu Pin College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Ji Hong College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Li Chao College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Tian Jingjing College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Wang Yifei College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Yu Ping College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 867-878 0920-1742 41:4<867 2015 41 10695 https://doi.org/10.1007/s10695-015-0053-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0053-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Pin College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Ji Hong College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Li Chao College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Tian Jingjing College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yifei College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 700 1- Yu Ping College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 867-878 0920-1742 41:4<867 2015 41 10695