caa a22 4500 606230238 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0061-x doi (NATIONALLICENCE)springer-10.1007/s10695-015-0061-x Egfl6 is involved in zebrafish notochord development [Elektronische Daten] [Xueqian Wang, Xin Wang, Wei Yuan, Renjie Chai, Dong Liu] The epidermal growth factor (EGF) repeat motif defines a superfamily of diverse protein involved in regulating a variety of cellular and physiological processes, such as cell cycle, cell adhesion, proliferation, migration, and neural development. Egfl6, an EGF protein, also named MAGE was first cloned in human tissue. Up to date, the study of zebrafish Egfl6 expression pattern and functional analysis of Egfl6 involved in embryonic development of vertebrate in vivo is thus far lacking. Here we reported that Egfl6 was involved in zebrafish notochord development. It was shown that Egfl6 mRNA was expressed in zebrafish, developing somites, fin epidermis, pharyngeal arches, and hindbrain region. Particularly the secreted Egfl6 protein was significantly accumulated in notochord. Loss of Egfl6 function in zebrafish embryos resulted in curved body with distorted notochord in the posterior trunk. It was observed that expression of all Notch ligand and receptors in notochord of 28hpf Egfl6 morphants was not affected, except notch2, which was up-regulated. We found that inhibition of Notch signaling by DAPT efficiently rescued notochord developmental defect of Egfl6 deficiency embryos. Springer Science+Business Media Dordrecht, 2015 Egfl6 nationallicence Notochord nationallicence Development nationallicence Zebrafish nationallicence Notch nationallicence Wang Xueqian Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut Wang Xin Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut Yuan Wei Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut Chai Renjie Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, 210096, Nanjing, China aut Liu Dong Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 961-969 0920-1742 41:4<961 2015 41 10695 https://doi.org/10.1007/s10695-015-0061-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0061-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Xueqian Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut NATIONALLICENCE 700 1- Wang Xin Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut NATIONALLICENCE 700 1- Yuan Wei Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut NATIONALLICENCE 700 1- Chai Renjie Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, 210096, Nanjing, China aut NATIONALLICENCE 700 1- Liu Dong Co-innovation Center of Neuroregeneration, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Qixiu Road 19, 226001, Nantong, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 961-969 0920-1742 41:4<961 2015 41 10695