caa a22 4500 606230246 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0056-7 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0056-7 Tissue distribution of olive flounder VDAC2 and its expression in fish cell lines [Elektronische Daten] [Aijun Lü, Xiucai Hu, Li Li, Chao Pei, Chao Zhang, Xianglin Cao, Xianghui Kong, Guoxing Nie, Xuejun Li, Jingfeng Sun] Voltage-dependent anion channel (VDAC) is located in the mitochondrial outer membrane, which plays a crucial role in regulating cell life and death. In this study, the tissue distribution of olive flounder Paralichthys olivaceus VDAC2 (PoVDAC2) was detected by quantitative real-time PCR and Western blot analysis. The qRT-PCR results showed that the expression level of PoVDAC2 was abundant in heart, muscle and gill tissues. Western blot analysis revealed a protein of 32kDa detected in all six tissues. Furthermore, a recombinant eukaryotic expression plasmid pEGFP-N3-PoVDAC2 was successfully constructed and transiently expressed the fusion protein in fish cell lines. Subcellular localization indicated that PoVDAC2-GFP was distributed in a punctate mitochondria-like pattern throughout the cytoplasm in flounder embryonic cells (FEC). The distribution of native VDAC2 in untransfected fish cells was also investigated by immunofluorescence microscopy. The punctate VDAC2 fluorescence signals of both FEC and EPC cells were identically observed in the cytoplasm but not in the nucleus. These results laid a foundation for investigating the functional relevance of VDAC response to pathogens in flounder. Springer Science+Business Media Dordrecht, 2015 VDAC nationallicence Olive flounder nationallicence Expression nationallicence Fish cell lines nationallicence Lü Aijun College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Hu Xiucai School of Life Sciences, Jiangsu Normal University, 221116, Xuzhou, China aut Li Li College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Pei Chao College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Zhang Chao College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Cao Xianglin College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Kong Xianghui College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Nie Guoxing College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Li Xuejun College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut Sun Jingfeng College of Fisheries, Tianjin Agricultural University, 300384, Tianjin, China aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 899-907 0920-1742 41:4<899 2015 41 10695 https://doi.org/10.1007/s10695-015-0056-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0056-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lü Aijun College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Hu Xiucai School of Life Sciences, Jiangsu Normal University, 221116, Xuzhou, China aut NATIONALLICENCE 700 1- Li Li College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Pei Chao College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Zhang Chao College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Cao Xianglin College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Kong Xianghui College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Nie Guoxing College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Li Xuejun College of Fisheries, Henan Normal University, 453007, Xinxiang, China aut NATIONALLICENCE 700 1- Sun Jingfeng College of Fisheries, Tianjin Agricultural University, 300384, Tianjin, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 899-907 0920-1742 41:4<899 2015 41 10695