caa a22 4500 606230254 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10695-015-0063-8 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0063-8 Effects of glucose, insulin and triiodothyroxine on leptin and leptin receptor expression and the effects of leptin on activities of enzymes related to glucose metabolism in grass carp ( Ctenopharyngodon idella ) hepatocytes [Elektronische Daten] [Rong-Hua Lu, Yi Zhou, Xiao-Chen Yuan, Xu-Fang Liang, Liu Fang, Xiao-Li Bai, Min Wang, Yu-Hua Zhao] Leptin is an important regulator of appetite and energy expenditure in mammals, but its role in fish metabolism control is poorly understood. Our previous studies demonstrated that leptin has an effect on the regulation of food intake and energy expenditure as well as lipid metabolism (stimulation of lipolysis and inhibition of adipogenesis) in the grass carp Ctenopharyngodon idella. To further investigate the role of leptin in fish, the effects of glucose, insulin and triiodothyroxine (T3) on the expression levels of leptin and leptin receptor (Lepr) and the effects of leptin on the activities of critical glucose metabolism enzymes in grass carp hepatocytes were evaluated in the present study. Our data indicated that leptin gene expression was induced by glucose in a dose-dependent manner, while Lepr gene expression exhibited a biphasic change. A high dose of insulin (100ng/mL) significantly up-regulated the expression of leptin and Lepr. Leptin expression was markedly up-regulated by a low concentration of T3 but inhibited by a high concentration of T3. T3 up-regulated Lepr expression in a dose-dependent manner. Together, these data suggest that leptin had a close relationship with three factors (glucose, insulin and T3) and might participate in the regulation of glucose metabolism in grass carp. In addition, we also found that leptin affected the activities of key enzymes that are involved in glucose metabolism, which might be mediated by insulin receptor substrate-phosphoinositol 3-kinase signaling. Springer Science+Business Media Dordrecht, 2015 Leptin nationallicence Leptin receptor nationallicence Glucose metabolism nationallicence Grass carp ( Ctenopharyngodon idella ) hepatocytes nationallicence G6Pase : Glucose 6-phosphatase nationallicence G6PD : Glucose-6-phosphate dehydrogenase nationallicence GK : Glucokinase nationallicence IRS-PI3K : Insulin receptor substrate-phosphatidylinositol-3-OH-kinase nationallicence JAK-STAT : Janus kinase-signal transducer and activator of transcription nationallicence Lepr : Leptin receptor nationallicence LPL : Lipoprotein lipase nationallicence ME : Malic enzyme nationallicence NPY : Neuropeptide Y nationallicence PEPCK : Phosphoenolpyruvate carboxykinase nationallicence PK : Pyruvate kinase nationallicence SCD1a : Stearoyl-CoA desaturase 1a nationallicence T3 : Triiodothyroxine nationallicence UCP2 : Uncoupling protein 2 nationallicence Lu Rong-Hua College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Zhou Yi College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Yuan Xiao-Chen College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Liang Xu-Fang College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Fang Liu College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Bai Xiao-Li College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Wang Min College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Zhao Yu-Hua College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 981-989 0920-1742 41:4<981 2015 41 10695 https://doi.org/10.1007/s10695-015-0063-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0063-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Rong-Hua College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Zhou Yi College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Yuan Xiao-Chen College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Liang Xu-Fang College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Fang Liu College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Bai Xiao-Li College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Wang Min College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 700 1- Zhao Yu-Hua College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, No. 1 Shizishan Street, Hongshan District, 430070, Wuhan, Hubei Province, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/4(2015-08-01), 981-989 0920-1742 41:4<981 2015 41 10695