caa a22 4500 606230297 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0081-6 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0081-6 A key gene of the small RNA pathway in the flounder, Paralichthys olivaceus [Elektronische Daten] identification and functional characterization of dicer [Yuanshuai Fu, Junling Zhang, Zhiyi Shi, Guyue Wang, Wejuan Li, Liang Jia] Dicer is critical for producing mature microRNAs (miRNAs) from precursor molecules and small interfering RNAs and plays an important role in controlling development and metabolism. In the present study, we cloned the flounder dicer gene, which is 6585 nucleotides (nt), including a 5′-untranslated region (UTR) of 231 nt, a 3′-UTR of 663 nt and an open reading frame of 5691 nt encoding a polypeptide of 1897 amino acids, and analyzed the conservation and expression pattern of dicer. The tissue distribution analysis indicated that dicer is abundantly expressed in the brain, heart, liver, spleen, stomach, kidney, gill, muscle, intestine and gonad of adult fish. Temporal expression analysis indicated that dicer mRNA is highly expressed during the embryonic and early larval stages, and exhibits low expression during the metamorphic stages. Treatment with thyroid hormone (TH) or thiourea indirectly or directly up-regulated dicer mRNA levels at 17 and 23dph, whereas treatment with TH down-regulated dicer mRNA levels at 36dph. The dicer-specific siRNA significantly down-regulated dicer mRNA and pol-let-7d levels, while pol-let-7d precursor levels were not differentially changed compared with the control (NC). These results demonstrated that dicer plays a key role in development and metabolism through the production of mature miRNAs, providing basic information for further studies concerning the role of dicer in Paralichthys olivaceus development. Springer Science+Business Media Dordrecht, 2015 Paralichthys olivaceus nationallicence Dicer nationallicence Thyroid hormone nationallicence Small interfering RNAs nationallicence MicroRNA nationallicence Fu Yuanshuai Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Zhang Junling Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Shi Zhiyi Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Wang Guyue Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Li Wejuan Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Jia Liang Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1221-1231 0920-1742 41:5<1221 2015 41 10695 https://doi.org/10.1007/s10695-015-0081-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0081-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fu Yuanshuai Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 700 1- Zhang Junling Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 700 1- Shi Zhiyi Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 700 1- Wang Guyue Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 700 1- Li Wejuan Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 700 1- Jia Liang Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, 999, Hu-Cheng-Huan Road, 201306, Lingang New City, Shangai, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1221-1231 0920-1742 41:5<1221 2015 41 10695