caa a22 4500 606230300 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0083-4 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0083-4 A comparison of single and multiple stressor protocols to assess acute stress in a coastal shark species, Rhizoprionodon terraenovae [Elektronische Daten] [Eric Hoffmayer, Jill Hendon, Glenn Parsons, William Driggers III, Matthew Campbell] Elasmobranch stress responses are traditionally measured in the field by either singly or serially sampling an animal after a physiologically stressful event. Although capture and handling techniques are effective at inducing a stress response, differences in protocols could affect the degree of stress experienced by an individual, making meaningful comparisons between the protocols difficult, if not impossible. This study acutely stressed Atlantic sharpnose sharks, Rhizoprionodon terraenovae, by standardized capture (rod and reel) and handling methods and implemented either a single or serial blood sampling protocol to monitor four indicators of the secondary stress response. Single-sampled sharks were hooked and allowed to swim around the boat until retrieved for a blood sample at either 0, 15, 30, 45, or 60min post-hooking. Serially sampled sharks were retrieved, phlebotomized, released while still hooked, and subsequently resampled at 15, 30, 45, and 60minintervals post-hooking. Blood was analyzed for hematocrit, and plasma glucose, lactate, and osmolality levels. Although both single and serial sampling protocols resulted in an increase in glucose, no significant difference in glucose level was found between protocols. Serially sampled sharks exhibited cumulatively heightened levels for lactate and osmolality at all time intervals when compared to single-sampled animals at the same time. Maximal concentration differences of 217.5, 9.8, and 41.6% were reported for lactate, osmolality, and glucose levels, respectively. Hematocrit increased significantly over time for the single sampling protocol but did not change significantly during the serial sampling protocol. The differences in resultant blood chemistry levels between implemented stress protocols and durations are significant and need to be considered when assessing stress in elasmobranchs. Springer Science+Business Media Dordrecht (outside the USA), 2015 Carcharhinidae nationallicence Glucose nationallicence Lactate nationallicence Osmolality nationallicence Hematocrit nationallicence Stress response nationallicence Hoffmayer Eric Department of Biology, University of Mississippi, 38677, University, MS, USA aut Hendon Jill Center for Fisheries Research and Development, Gulf Coast Research Laboratory, The University of Southern Mississippi, 703 East Beach Drive, 39564, Ocean Springs, MS, USA aut Parsons Glenn Department of Biology, University of Mississippi, 38677, University, MS, USA aut Driggers III William National Marine Fisheries Service, Southeast Fisheries Science Center, Mississippi Laboratories, 3209 Frederic Street, 39567, Pascagoula, MS, USA aut Campbell Matthew National Marine Fisheries Service, Southeast Fisheries Science Center, Mississippi Laboratories, 3209 Frederic Street, 39567, Pascagoula, MS, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1253-1260 0920-1742 41:5<1253 2015 41 10695 https://doi.org/10.1007/s10695-015-0083-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0083-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hoffmayer Eric Department of Biology, University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- Hendon Jill Center for Fisheries Research and Development, Gulf Coast Research Laboratory, The University of Southern Mississippi, 703 East Beach Drive, 39564, Ocean Springs, MS, USA aut NATIONALLICENCE 700 1- Parsons Glenn Department of Biology, University of Mississippi, 38677, University, MS, USA aut NATIONALLICENCE 700 1- Driggers III William National Marine Fisheries Service, Southeast Fisheries Science Center, Mississippi Laboratories, 3209 Frederic Street, 39567, Pascagoula, MS, USA aut NATIONALLICENCE 700 1- Campbell Matthew National Marine Fisheries Service, Southeast Fisheries Science Center, Mississippi Laboratories, 3209 Frederic Street, 39567, Pascagoula, MS, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1253-1260 0920-1742 41:5<1253 2015 41 10695