caa a22 4500 606230319 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0071-8 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0071-8 Identification and expression of SRF targeted by miR-133a during early development of Paralichthys olivaceus [Elektronische Daten] [Yanfang Su, Yuanshuai Fu, Hongmei Zhang, Zhiyi Shi, Junling Zhang, Lina Gao] Serum response factor (SRF) is a MADS-box transcription factor that regulates the expression of genes involved in development, metabolism, cell proliferation, and differentiation. In the present study, we cloned the full-length SRF cDNA which includes the coding region of 1503bp, a 573-bp 5′untranslated region (UTR) and a 400-bp 3′-UTR. The deduced 501 amino acid sequence of the SRF protein contained a MADS domain and NLS at the N terminus, similar to other organisms, and it also is highly phylogenetically conserved. SRF mRNA is ubiquitously expressed in various tissues, with the highest level in the kidneys, and it is also highly expressed during the embryonic and metamorphic stages. During metamorphosis, the SRF mRNA levels are down-regulated by exogenous thyroid hormone (TH) at 17dph and by thiourea (TU) at 29, 36, and 41dph, whereas SRF mRNA levels were significantly up-regulated by the added exogenous TH to the TU-treated larvae at 41dph, which indicates that thyroid hormone is essential for expression of SRF mRNA, so, higher levels of TH did not result in changes of SRF mRNA levels, while TH deficiency or inhibited by the non-specific TU toxicity cause down-regulation of SRF mRNA, which indicated that TH can indirectly affect the SRF mRNA levels. Meanwhile, using a luciferase reporter assay, we verified that SRF is a common target gene of miR-133a which is a muscle-specific microRNA (miRNA), which indicated that SRF may be involved in the signaling pathway of miRNA that regulates muscle development. Springer Science+Business Media Dordrecht, 2015 Paralichthys olivaceus nationallicence SRF nationallicence Metamorphosis nationallicence miR-133a nationallicence Target gene nationallicence Su Yanfang Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Fu Yuanshuai Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Zhang Hongmei Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Shi Zhiyi Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Zhang Junling Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Gao Lina Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1093-1104 0920-1742 41:5<1093 2015 41 10695 https://doi.org/10.1007/s10695-015-0071-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0071-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Su Yanfang Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Fu Yuanshuai Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Hongmei Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Shi Zhiyi Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Junling Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 700 1- Gao Lina Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, 201306, Shanghai, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1093-1104 0920-1742 41:5<1093 2015 41 10695