caa a22 4500 606230327 CHVBK 20210128101213.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0087-0 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0087-0 Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp ( Cyprinus carpio var. Jian ) [Elektronische Daten] [HuaTao Li, Lin Feng, WeiDan Jiang, Yang Liu, Jun Jiang, YongAn Zhang, Pei Wu, XiaoQiu Zhou] There are young erythrocytes and mature erythrocytes in the peripheral blood of fish. The present study explored the apoptosis in hydroxyl radical (·OH)-induced young and mature erythrocytes of Jian carp (Cyprinus carpio var. Jian). Carp erythrocytes from the peripheral blood were separated into the young fraction, the intermediate fraction and the mature fraction using fixed-angle centrifugation. The erythrocytes in three age fractions were treated with the caspase inhibitors (zVAD-fmk) in physiological carp saline (PCS) or Ca2+-free PCS in the presence of 40μM FeSO4/20μM H2O2. The results showed that the ·OH-induced reactive oxygen species (ROS) generation, phosphatidylserine (PS) exposure and DNA fragmentation are caspase dependent in carp erythrocytes. Furthermore, the ROS generation, PS exposure and DNA fragmentation in the more young fraction are more dependent on the caspase activity. This suggested that the caspases are involved in the ·OH-induced apoptosis in the young erythrocytes of fish. Results also indicated that Ca2+ is involved in ·OH-induced calpain activation, PS exposure and DNA fragmentation in carp erythrocytes. Moreover, the calpain activation, DNA fragmentation and PS exposure in the more mature fraction are more dependent on the levels of Ca2+. This revealed that ·OH-induced apoptosis is Ca2+ dependent in the mature erythrocytes of fish. Taken together, there might be two apoptosis pathways in fish erythrocytes: one is the caspase-dependent apoptosis in the young erythrocytes and the other is the Ca2+-involved apoptosis in the mature erythrocytes. Springer Science+Business Media Dordrecht, 2015 Fish erythrocyte nationallicence Apoptosis nationallicence Ca2+ nationallicence Caspase nationallicence PS exposure nationallicence DNA fragmentation nationallicence Li HuaTao Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Feng Lin Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Jiang WeiDan Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Liu Yang Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Jiang Jun Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Zhang YongAn Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut Wu Pei Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Zhou XiaoQiu Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1305-1319 0920-1742 41:5<1305 2015 41 10695 https://doi.org/10.1007/s10695-015-0087-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0087-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li HuaTao Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Feng Lin Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Jiang WeiDan Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Liu Yang Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Jiang Jun Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Zhang YongAn Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China aut NATIONALLICENCE 700 1- Wu Pei Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 700 1- Zhou XiaoQiu Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1305-1319 0920-1742 41:5<1305 2015 41 10695