caa a22 4500 60623036X CHVBK 20210128101214.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0080-7 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0080-7 Metabolic responses of the Antarctic fishes Notothenia rossii and Notothenia coriiceps to sewage pollution [Elektronische Daten] [Edson Rodrigues Jr., Mariana Feijó-Oliveira, Cecília Suda, Gannabathula Vani, Lucélia Donatti, Edson Rodrigues, Helena Lavrado] The present study aimed to assess the sewage effects of the Brazilian Antarctic Station Comandante Ferraz, Admiralty Bay, King George Island, on the hepatic metabolism (energetic, antioxidant, and arginase levels) and levels of plasma constituents of two Antarctic fish species Notothenia rossii and N. coriiceps. The bioassays were conducted under controlled temperature (0°C) and salinity (35psu), exposing the fish for 96h, to sewage effluent diluted in seawater to 0.5% (v/v). Liver homogenates were tested for the specific activities of the enzymes glucose-6-phosphatase (G6Pase), glycogen phosphorylase (GPase), hexokinase, citrate synthase, lactate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutathione reductase, catalase, and arginase. Plasma levels of glucose, triacylglycerides, cholesterol, total protein, albumin, chloride, magnesium, calcium, and inorganic phosphate were also determined. In N. rossii, the decrease in citrate synthase and the increase in G6Pase and GPase suggested that the sewage effluent activated glycogenolysis and hepatic gluconeogenesis, whereas is N. coriiceps, only G6Pase levels were increased. In N. rossii, sewage effluent induced hypertriglyceridemia without modulating glucose plasma levels, in contrast to N. coriiceps, which developed hypoglycemia without elevating plasma triglyceride levels. The decrease in glutathione reductase levels in N. coriiceps and in superoxide dismutase and catalase in N. rossii suggest that these two species are susceptible to oxidative stress stemming from the production of reactive oxygen species. An increase in magnesium in N. rossii and a decrease in N. coriiceps showed that sewage effluent compromised the control of plasma levels of this cation. Although phylogenetically close, both species of Antarctic fish exhibited different metabolic responses to the sewage effluent, with N. coriiceps showing greater susceptibility to the toxic effects of the pollutants. The present study suggests that the biochemical responses of these two species are potential indicators of metabolic changes caused by sewage effluents. Springer Science+Business Media Dordrecht, 2015 Antarctica nationallicence Sewage nationallicence Liver nationallicence Energy metabolism nationallicence Oxidative defense nationallicence Rodrigues Jr. Edson Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut Feijó-Oliveira Mariana Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut Suda Cecília Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut Vani Gannabathula Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut Donatti Lucélia Departamento of Cell Biology, Federal University of Paraná, Centro Politécnico s/No, 81.530-130, Curitiba, PR, Brazil aut Rodrigues Edson Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut Lavrado Helena Departamento of Marine Biology, Federal University of Rio de Janeiro, 373 - CCS - Bloco A - sala 89, 21.941-902, Rio de Janeiro, RJ, Brazil aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1205-1220 0920-1742 41:5<1205 2015 41 10695 https://doi.org/10.1007/s10695-015-0080-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0080-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rodrigues Jr Edson Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut NATIONALLICENCE 700 1- Feijó-Oliveira Mariana Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut NATIONALLICENCE 700 1- Suda Cecília Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut NATIONALLICENCE 700 1- Vani Gannabathula Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut NATIONALLICENCE 700 1- Donatti Lucélia Departamento of Cell Biology, Federal University of Paraná, Centro Politécnico s/No, 81.530-130, Curitiba, PR, Brazil aut NATIONALLICENCE 700 1- Rodrigues Edson Institute of Basic Bioscience, University of Taubaté, Av. Tiradentes, 500 - Centro, 12.030-180, Taubaté, SP, Brazil aut NATIONALLICENCE 700 1- Lavrado Helena Departamento of Marine Biology, Federal University of Rio de Janeiro, 373 - CCS - Bloco A - sala 89, 21.941-902, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1205-1220 0920-1742 41:5<1205 2015 41 10695