caa a22 4500 606230394 CHVBK 20210128101214.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0076-3 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0076-3 Effects of glutamate on growth, antioxidant capacity, and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes [Elektronische Daten] [Jun Jiang, Dan Shi, Xiao-Qiu Zhou, Long Yin, Lin Feng, Yang Liu, Wei-Dan Jiang, Ye Zhao] The present study explored the effects of glutamate (Glu) on the growth, antioxidant capacity, and gene expression of NF-E2-related nuclear factor 2 (Nrf2) signaling molecule in enterocytes of Jian carp (Cyprinus carpio var. Jian). The enterocytes were incubated in media containing 0, 2, 4, 6, 8, and 10mM/L Glu for 96h. The results showed that Glu could promote fish enterocytes proliferation and differentiation. Additionally, activities of alkaline phosphatase, Na+, K+-ATPase, γ-glutamyl transpeptidase, and creatine kinase were significantly improved with the increase in Glu level up to 6mM/L. Lactic acid dehydrogenase activity and malondialdehyde content in the medium and cellular protein carbonyls were depressed by Glu. Moreover, optimum Glu significantly enhanced glutathione content and the activities and gene expression of catalase, glutathione reductase, and glutathione peroxidase in enterocytes. Finally, the expression level of Nrf2 in enterocytes was significantly elevated by appropriate Glu content in the medium. Furthermore, optimum Glu significantly decreased Kelch-like ECH-associated protein 1 mRNA level in enterocytes. In conclusion, Glu improved the proliferation, function, and antioxidant capacity and regulated antioxidant-related signaling molecule expression of fish enterocytes. Springer Science+Business Media Dordrecht, 2015 Growth nationallicence Antioxidant capacity nationallicence Signaling molecule nationallicence Enterocytes nationallicence Glutamate nationallicence Jiang Jun College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut Shi Dan College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut Zhou Xiao-Qiu Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut Yin Long College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut Feng Lin Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut Liu Yang Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut Jiang Wei-Dan Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut Zhao Ye College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1143-1153 0920-1742 41:5<1143 2015 41 10695 https://doi.org/10.1007/s10695-015-0076-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0076-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Jun College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut NATIONALLICENCE 700 1- Shi Dan College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Xiao-Qiu Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yin Long College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut NATIONALLICENCE 700 1- Feng Lin Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yang Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Wei-Dan Animal Nutrition Institute, Sichuan Agricultural University, 611130, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Ye College of Animal Science and Technology, Sichuan Agricultural University, No. 211, Huimin Road, Wenjiang District, 611130, Chengdu, Sichuan Province, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1143-1153 0920-1742 41:5<1143 2015 41 10695