caa a22 4500 606230408 CHVBK 20210128101214.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0070-9 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0070-9 Development of digestive tract and enzyme activities during the early ontogeny of the tropical gar Atractosteus tropicus [Elektronische Daten] [C. Frías-Quintana, G. Márquez-Couturier, C. Alvarez-González, D. Tovar-Ramírez, H. Nolasco-Soria, M. Galaviz-Espinosa, R. Martínez-García, S. Camarillo-Coop, R. Martínez-Yañez, E. Gisbert] Changes in digestive enzyme activity and histology were studied in Atractosteus tropicus embryos, larvae and juvenile periods. Alkaline protease, chymotrypsin, carboxypeptidase A, lipase and α-amylase were detected in all periods and gradually increased until reaching the maximum peak in juveniles; meanwhile, acid protease was first detected at 5days after hatching (dah) when first feeding started and trypsin and leucine aminopeptidase activities were detected from 19 dah, their values being increased gradually until reaching a maximum value at 31 dah. Acid and alkaline phosphatase activities increased from yolk-sac absorption (3 dah) until day 31 after hatching. Zymogram for acid protease showed two bands in active forms (0.4 and 0.5 Rfs) from day 5 after hatching and a third protease form (0.3 Rf) that appears at 31 dah. Two active forms (26.3 and 24.9kDa) were detected using SDS-PAGE alkaline proteases zymogram at 5 dah, and an additional active form (44.1 kDa) was detected at 7 dah. Regarding the histological development of the digestive system, the exocrine pancreas containing zymogen granules was already visible at 3 dah, whereas at 5 dah first gastric glands were already detected in the stomach. Between 7 and 9 dah, the digestive tract of A. tropicus resembled that of a juvenile specimen with a well-developed and short oesophagus, stomach divided into a glandular and non-glandular (pyloric) stomach, folded intestine with pyloric caeca and a well-developed spiral valve (posterior intestine). Considering this, larvae of A. tropicus are capable of digesting several foods from yolk absorption (3 dah), maximizing its activities at 15 dah, age at which the organisms maximize its capability to absorb nutrients from diets provided. Springer Science+Business Media Dordrecht, 2015 α-Amylase nationallicence Atractosteus tropicus nationallicence Phosphatase nationallicence Lipase nationallicence Ontogeny nationallicence Proteases nationallicence Frías-Quintana C. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut Márquez-Couturier G. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut Alvarez-González C. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut Tovar-Ramírez D. Centro de Investigaciones Biológicas del Noroeste S.C., Av. Instituto Politécnico Nacional 195, Colonia Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico aut Nolasco-Soria H. Centro de Investigaciones Biológicas del Noroeste S.C., Av. Instituto Politécnico Nacional 195, Colonia Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico aut Galaviz-Espinosa M. Facultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), PO Box 76, 22860, Ensenada, B.C., Mexico aut Martínez-García R. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut Camarillo-Coop S. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut Martínez-Yañez R. División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Ex Hacienda El Copal, Irapuato, Guanajuato, Mexico aut Gisbert E. IRTA - Sant Carles de la Ràpita, Crta. Poble Nou km 5.5, 43540, Sant Carles de la Rapita, Tarragona, Spain aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1075-1091 0920-1742 41:5<1075 2015 41 10695 https://doi.org/10.1007/s10695-015-0070-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0070-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frías-Quintana C. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut NATIONALLICENCE 700 1- Márquez-Couturier G. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut NATIONALLICENCE 700 1- Alvarez-González C. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut NATIONALLICENCE 700 1- Tovar-Ramírez D. Centro de Investigaciones Biológicas del Noroeste S.C., Av. Instituto Politécnico Nacional 195, Colonia Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico aut NATIONALLICENCE 700 1- Nolasco-Soria H. Centro de Investigaciones Biológicas del Noroeste S.C., Av. Instituto Politécnico Nacional 195, Colonia Playa Palo de Santa Rita Sur, 23096, La Paz, Baja California Sur, Mexico aut NATIONALLICENCE 700 1- Galaviz-Espinosa M. Facultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), PO Box 76, 22860, Ensenada, B.C., Mexico aut NATIONALLICENCE 700 1- Martínez-García R. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut NATIONALLICENCE 700 1- Camarillo-Coop S. Laboratorio de Acuicultura Tropical, DACBIOL-UJAT, Carretera Villahermosa-Cárdenas KM 0.5, 86139, Villahermosa, Tabasco, Mexico aut NATIONALLICENCE 700 1- Martínez-Yañez R. División de Ciencias de la Vida, Campus Irapuato-Salamanca, Universidad de Guanajuato, Ex Hacienda El Copal, Irapuato, Guanajuato, Mexico aut NATIONALLICENCE 700 1- Gisbert E. IRTA - Sant Carles de la Ràpita, Crta. Poble Nou km 5.5, 43540, Sant Carles de la Rapita, Tarragona, Spain aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1075-1091 0920-1742 41:5<1075 2015 41 10695