caa a22 4500 606230467 CHVBK 20210128101214.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0090-5 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0090-5 Effects of a Dissostichus mawsoni - CaM recombinant proteins feed additive on the juvenile orange-spotted grouper ( Epinephelus coioides ) under the acute low temperature challenge [Elektronische Daten] [Sheng-Wei Luo, Wei-Na Wang, Luo Cai, Zeng-Hua Qi, Cong Wang, Yuan Liu, Chang-Lian Peng, Liang-Biao Chen] The effects of Dissostichus mawsoni-Calmodulin (Dm-CaM) on growth performance, enzyme activities, respiratory burst, MDA level and immune-related gene expressions of the orange-spotted grouper (Epinephelus coioides) exposed to the acute low temperature stress were evaluated. The commercial diet supplemented with Dm-CaM protein was fed to the groupers for 6weeks. No significant difference was observed in the specific growth rates, weight gains and survivals. After the feeding trial, the groupers were exposed to acute low temperature challenge. The groupers fed with Dm-CaM additive diet showed a significant decrease in the respiratory burst activity, while the blood cell number increased significantly at 25°C by comparing with the control and additive control group. The enzymatic activity of SOD, ACP and ALP increased significantly in Dm-CaM additive group, while MDA level maintained stable with the lowest value. qRT-PCR analysis indicated that the up-regulated transcript expressions of CaM, C3, SOD2, LysC and HSPA4 were observed in Dm-CaM additive group. These results indicated that Dm-CaM additive diet may regulate the grouper immune response to the acute low temperature challenge. Springer Science+Business Media Dordrecht, 2015 Epinephelus coioides nationallicence Acute low temperature challenge nationallicence Calmodulin nationallicence Oxidative stress nationallicence Gene expression nationallicence Dietary feed additive nationallicence Luo Sheng-Wei Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Wang Wei-Na Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Cai Luo Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Qi Zeng-Hua Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Wang Cong College of Food Science and Technology, Agricultural University of Hebei, 071001, Baoding, People's Republic of China aut Liu Yuan Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Peng Chang-Lian Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Chen Liang-Biao Key Laboratory of Aquatic Resources and Utilization, MOE, China, College of Fisheries and Life Sciences, Shanghai Ocean University, 201306, Shanghai, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1345-1358 0920-1742 41:5<1345 2015 41 10695 https://doi.org/10.1007/s10695-015-0090-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0090-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luo Sheng-Wei Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wei-Na Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cai Luo Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Qi Zeng-Hua Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Cong College of Food Science and Technology, Agricultural University of Hebei, 071001, Baoding, People's Republic of China aut NATIONALLICENCE 700 1- Liu Yuan Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Peng Chang-Lian Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Liang-Biao Key Laboratory of Aquatic Resources and Utilization, MOE, China, College of Fisheries and Life Sciences, Shanghai Ocean University, 201306, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1345-1358 0920-1742 41:5<1345 2015 41 10695