caa a22 4500 606230483 CHVBK 20210128101214.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10695-015-0079-0 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0079-0 Effects of dietary fatty acids on mitochondrial phospholipid compositions, oxidative status and mitochondrial gene expression of zebrafish at different ages [Elektronische Daten] [M. Betancor, P. Almaida-Pagán, A. Hernández, D. Tocher] Mitochondrial decay is generally associated with impairment in the organelle bioenergetics function and increased oxidative stress, and it appears that deterioration of mitochondrial inner membrane phospholipids (PL) and accumulation of mitochondrial DNA (mtDNA) mutations are among the main mechanisms involved in this process. In the present study, mitochondrial membrane PL compositions, oxidative status (TBARS content and SOD activity) and mtDNA gene expression of muscle and liver were analyzed in zebrafish fed two diets with lipid supplied either by rapeseed oil (RO) or a blend 60:40 of RO and DHA500 TG oil (DHA). Two feeding trials were performed using zebrafish from the same population of two ages (8 and 21months). Dietary FA composition affected fish growth in 8-month-old animals, which could be related to an increase in stress promoted by diet composition. Lipid peroxidation was considerably higher in mitochondria of 8-month-old zebrafish fed the DHA diet than in animals fed the RO diet. This could indicate higher oxidative damage to mitochondrial lipids, very likely due to increased incorporation of DHA in PL of mitochondrial membranes. Lipids would be among the first molecules affected by mitochondrial reactive oxygen species, and lipid peroxidation could propagate oxidative reactions that would damage other molecules, including mtDNA. Mitochondrial lipid peroxidation and gene expression of 21-month-old fish showed lower responsiveness to diet composition than those of younger fish. Differences found in the effect of diet composition on mitochondrial lipids between the two age groups could be indicating age-related changes in the ability to maintain structural homeostasis of mitochondrial membranes. Springer Science+Business Media Dordrecht, 2015 Diet nationallicence Fatty acid nationallicence Mitochondria nationallicence Zebrafish nationallicence Oxidative stress nationallicence Phospholipid nationallicence B2M : β-2-Microglobulin nationallicence BACT : β-Actin nationallicence BHT : Butylated hydroxytoluene nationallicence cDNA : Complementary DNA nationallicence CL : Cardiolipin nationallicence COX : Cytochrome c oxidase complex nationallicence DHA : Docosahexaenoic acid nationallicence ETC : Electron transport chain nationallicence FA : Fatty acid nationallicence FAME : Fatty acid methyl esters nationallicence HP-TLC : High-performance thin-layer chromatography nationallicence LA : Linoleic acid nationallicence LC-PUFA : Long-chain polyunsaturated fatty acid nationallicence MIM : Mitochondrial inner membrane nationallicence mtDNA : Mitochondrial DNA nationallicence MUFA : Monounsaturated fatty acids nationallicence NAC : No-amplification control nationallicence ND : NADH-coenzyme Q oxidoreductase complex nationallicence NTC : No-template control nationallicence OA : Oleic acid nationallicence PC : Phosphatidylcholine nationallicence PE : Phosphatidylethanolamine nationallicence PI : Phosphatidylinositol nationallicence PIn : Peroxidation index nationallicence PL : Phospholipid nationallicence PS : Phosphatidylserine nationallicence PUFA : Polyunsaturated fatty acid nationallicence qPCR : Quantitative PCR nationallicence RO : Rapeseed oil nationallicence ROS : Reactive oxygen species nationallicence SFA : Saturated fatty acids nationallicence SM : Sphingomyelin nationallicence RT-PCR : Real-time PCR nationallicence SEM : Standard error of the mean nationallicence SGR : Specific growth rate nationallicence SOD : Superoxide dismutase nationallicence TBARS : Thiobarbituric acid reactive substances nationallicence TBA : Thiobarbituric acid nationallicence TCA : Trichloroacetic acid nationallicence TLC : Thin-layer chromatography nationallicence Tm : Melting temperature nationallicence Betancor M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Hernández A. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1187-1204 0920-1742 41:5<1187 2015 41 10695 https://doi.org/10.1007/s10695-015-0079-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0079-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Betancor M. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- Almaida-Pagán P. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- Hernández A. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 700 1- Tocher D. School of Natural Sciences, Institute of Aquaculture, University of Stirling, FK9 4LA, Stirling, Scotland, UK aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/5(2015-10-01), 1187-1204 0920-1742 41:5<1187 2015 41 10695