caa a22 4500 606230491 CHVBK 20210128101214.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-015-0027-z doi (NATIONALLICENCE)springer-10.1007/s10695-015-0027-z Differences in growth, fillet quality, and fatty acid metabolism-related gene expression between juvenile male and female rainbow trout [Elektronische Daten] [Meghan Manor, Beth Cleveland, P. Brett Kenney, Jianbo Yao, Tim Leeds] Sexual maturation occurs at the expense of stored energy and nutrients, including lipids; however, little is known regarding sex effects on nutrient regulatory mechanisms in rainbow trout prior to maturity. Thirty-two, 14-month-old, male and female rainbow trout were sampled for growth, carcass yield, fillet composition, and gene expression of liver, white muscle, and visceral adipose tissue. Growth parameters, including gonadosomatic index, were not affected by sex. Females had higher percent separable muscle yield, but there were no sex effects on fillet proximate composition. Fillet shear force indicated females produce firmer fillets than males. Male livers had greater expression of three cofactors within the mTOR signaling pathway that act to inhibit TORC1 assembly; mo25, rictor, and pras40. Male liver also exhibited increased expression of β-oxidation genes cpt1b and ehhadh. These findings are indicative of increased mitochondrial β-oxidation in male liver. Females exhibited increased expression of the mTOR cofactor raptor in white muscle and had higher expression levels of several genes within the fatty acid synthesis pathway, including gpat, srebp1, scd1, and cd36. Female muscle also had increased expression of β-oxidation genes cpt1d and cpt2. Increased expression of both fatty acid synthesis and β-oxidation genes suggests female muscle may have greater fatty acid turnover. Differences between sexes were primarily associated with variation of gene expression within the mTOR signaling pathway. Overall, data suggest there is differential regulation of gene expression in male and female rainbow trout tissues prior to the onset of sexual maturity that may lead to nutrient repartitioning during maturation. Springer Science+Business Media Dordrecht, 2015 Salmonids nationallicence PPAR nationallicence mTOR nationallicence Fatty acids nationallicence Fillet quality nationallicence Manor Meghan Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut Cleveland Beth National Center for Cool and Cold Water Aquaculture, ARS/USDA, 11861 Leetown Road, 25430, Kearneysville, WV, USA aut Brett Kenney P. Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut Yao Jianbo Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut Leeds Tim National Center for Cool and Cold Water Aquaculture, ARS/USDA, 11861 Leetown Road, 25430, Kearneysville, WV, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 533-547 0920-1742 41:2<533 2015 41 10695 https://doi.org/10.1007/s10695-015-0027-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0027-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manor Meghan Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Cleveland Beth National Center for Cool and Cold Water Aquaculture, ARS/USDA, 11861 Leetown Road, 25430, Kearneysville, WV, USA aut NATIONALLICENCE 700 1- Brett Kenney P. Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Yao Jianbo Division of Animal and Nutritional Sciences, Davis College of Agriculture, Forestry, and Consumer Sciences, West Virginia University, 1042 Agricultural Sciences Building, PO Box 6108, 26505-6108, Morgantown, WV, USA aut NATIONALLICENCE 700 1- Leeds Tim National Center for Cool and Cold Water Aquaculture, ARS/USDA, 11861 Leetown Road, 25430, Kearneysville, WV, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 533-547 0920-1742 41:2<533 2015 41 10695