caa a22 4500 606230513 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-014-9986-8 doi (NATIONALLICENCE)springer-10.1007/s10695-014-9986-8 Effects of lipid-lowering pharmaceutical clofibrate on lipid and lipoprotein metabolism of grass carp ( Ctenopharyngodon idellal Val.) fed with the high non-protein energy diets [Elektronische Daten] [Xiaoze Guo, Xu-Fang Liang, Liu Fang, Xiaochen Yuan, Yi Zhou, Shan He, Dan Shen] This study investigated the effects of clofibrate treatment on blood lipids, hepatic enzyme activities and relative expression of genes involved in lipid metabolism of grass carp fed with high non-protein energy diets. For that purpose, five diets were formulated: a commercial-like diet (Control), a high-carbohydrate diet (HC), a high-fat diet (HF) and two diets identical to the HC and HF diets, but supplemented with 1.25gkg−1 clofibrate (HC+Clo and HF+Clo diets). Grass carp fed the HC and HF diet exhibited increases in blood lipids and body fat compared with the control group after 4weeks. In the clofibrate treatment groups, there was a marked decrease in triacylglycerol and cholesterol concentrations of plasma, and total lipids of the whole body, mesentery adipose tissue and liver tissue. Fish treated with clofibrate exhibited increased hepatic acyl-CoA oxidase activity, but did not show any changes in carnitine palmitoyltransferase (CPT) I activity compared with HC and HF diets without clofibrate. Clofibrate treatment had no effect on peroxisome proliferator-activated receptor alpha and CPT I mRNA expression. However, there was an increase in lipoprotein lipase expression in the clofibrate-treated groups. In addition, the relative mRNA expression levels of hepatic de novo lipogenic enzymes (fatty acid synthetase and acetyl coenzyme-A carboxylase) were significantly higher in the fish fed the HC diet than those of other groups, and clofibrate inhibited this increase. These results suggest that clofibrate has the hypolipidaemic effects and affects lipid metabolism in grass carp. Springer Science+Business Media Dordrecht, 2014 Grass carp nationallicence Clofibrate nationallicence High non-protein energy diets nationallicence Lipid metabolism nationallicence Gene expression nationallicence Guo Xiaoze College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Liang Xu-Fang College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Fang Liu College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Yuan Xiaochen College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Zhou Yi College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut He Shan College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Shen Dan College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 331-343 0920-1742 41:2<331 2015 41 10695 https://doi.org/10.1007/s10695-014-9986-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9986-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Xiaoze College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Liang Xu-Fang College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Fang Liu College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Yuan Xiaochen College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhou Yi College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- He Shan College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Shen Dan College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Hubei Collaborative Innovation Center for Freshwater Aquaculture, No.1, Shizishan Street, Hongshan District, 430070, Wuhan, Hubei, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 331-343 0920-1742 41:2<331 2015 41 10695