caa a22 4500 606230521 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-014-9990-z doi (NATIONALLICENCE)springer-10.1007/s10695-014-9990-z Identification and expression of GnRH2 and GnRH3 in the black sea bass ( Centropristis striata ), a hermaphroditic teleost [Elektronische Daten] [Scott Morin, Wayne Decatur, Timothy Breton, Timothy Marquis, Mary Hayes, David Berlinsky, Stacia Sower] We cloned two cDNAs for two gonadotropin-releasing hormones, GnRH2 (chicken GnRH-II) and GnRH3 (salmon GnRH), respectively, from the black sea bass (Centropristis striata). Black sea bass are protogynous hermaphroditic teleosts that change from females to males between 2 and 5years of age. Similar to other GnRH precursors, the precursors of black sea bass GnRH2 and GnRH23 consisted of a signal peptide, decapeptide, a downstream processing site, and a GnRH-associated peptide. Our analyses failed to identify GnRH1. GnRH3 precursor transcript was more widely distributed in a variety of tissues compared with GnRH2. Further examination of GnRH expression and gonadal histology was done in black sea bass from three different size groups: small (11.4-44.1g), medium (179.4-352.2g) and large (393.8-607.3g). Interestingly, GnRH3 expression occurred only in the pituitaries of males in the small and medium groups compared with expression of GnRH2. Future functional studies of the sea bass GnRHs will be valuable in elucidating the potential underlying neuroendocrine mechanisms of black sea bass reproduction and may ultimately contribute to management advances in this commercially important fish. Springer Science+Business Media Dordrecht, 2014 Gonadotropin-releasing hormone nationallicence Teleosts nationallicence Sex change nationallicence Reproduction nationallicence Protogynous nationallicence Brain nationallicence Morin Scott Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Decatur Wayne Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Breton Timothy Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Marquis Timothy Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Hayes Mary Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Berlinsky David Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Sower Stacia Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 383-395 0920-1742 41:2<383 2015 41 10695 https://doi.org/10.1007/s10695-014-9990-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9990-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morin Scott Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Decatur Wayne Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Breton Timothy Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Marquis Timothy Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Hayes Mary Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Berlinsky David Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 700 1- Sower Stacia Center for Molecular and Comparative Endocrinology, University of New Hampshire, 316 Rudman Hall, 46 College Road, 03824-3544, Durham, NH, USA aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 383-395 0920-1742 41:2<383 2015 41 10695