caa a22 4500 606230548 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-015-0029-x doi (NATIONALLICENCE)springer-10.1007/s10695-015-0029-x Germ cell-specific expression of dead end ( dnd ) in rare minnow ( Gobiocypris rarus ) [Elektronische Daten] [Jundan Duan, Gongqing Feng, Pei Chang, Xueyan Zhang, Qingchun Zhou, Xueping Zhong, Chao Qi, Songguang Xie, Haobin Zhao] Rare minnow (Gobiocypris rarus) is an emerging model fish in China, and the development of its gonads is still elusive. Germ cell-specific genes are conserved in animals. Dead end (Dnd) was first documented as a germ granule component in zebrafish. Here, we report the cloning and expression profile of dnd in rare minnow. RT-PCR results showed that dnd is expressed specifically in the gonads of both sexes, is maternal in origin and is expressed continuously during embryogenesis. Dnd mRNA could be detected exclusively in the germ cells of the testis and ovary. Temporal expression of dnd mRNA is similar to that of vasa and dnd in zebrafish during embryogenesis. Taken together, dnd mRNA is restricted to the germ cells of rare minnow. Springer Science+Business Media Dordrecht, 2015 Rare minnow nationallicence Germ cells nationallicence Primordial germ cells nationallicence dead end nationallicence Testis nationallicence Ovary nationallicence Gene expression nationallicence Embryonic development nationallicence AA : Amino acids nationallicence BCIP : 5-bromo-4-chloro-3-indolyl phosphate nationallicence bp : Base pairs nationallicence cDNA : Complementary DNA nationallicence DIG : Digoxigenin nationallicence dnd : dead end nationallicence DSRM : Double-stranded RNA recognition motif nationallicence hpf : Hours post-fertilization nationallicence LATS2 : Large tumor suppressor kinase 2 nationallicence NBT : Nitroblue tetrazolium nationallicence ORF : Open reading frame nationallicence PBS : Phosphate buffered saline nationallicence PCR : Polymerase chain reaction nationallicence PGC : Primordial germ cell nationallicence RACE : Rapid amplification of cDNA ends nationallicence RBD : RNA-binding domain nationallicence RNP : Ribonucleoprotein domain nationallicence RRM : RNA recognition motifs nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence UTR : Untranslated region nationallicence Duan Jundan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Feng Gongqing Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Chang Pei Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Zhang Xueyan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Zhou Qingchun Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Zhong Xueping Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Qi Chao Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Xie Songguang Key Laboratory of Aquatic Biodiversity and Resource Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, China aut Zhao Haobin Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 561-571 0920-1742 41:2<561 2015 41 10695 https://doi.org/10.1007/s10695-015-0029-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0029-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Duan Jundan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Feng Gongqing Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Chang Pei Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Zhang Xueyan Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Zhou Qingchun Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Zhong Xueping Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Qi Chao Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 700 1- Xie Songguang Key Laboratory of Aquatic Biodiversity and Resource Conservation, Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, Hubei, China aut NATIONALLICENCE 700 1- Zhao Haobin Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 430079, Wuhan, China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 561-571 0920-1742 41:2<561 2015 41 10695