caa a22 4500 606230556 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-014-9993-9 doi (NATIONALLICENCE)springer-10.1007/s10695-014-9993-9 Effect of the addition of six antioxidants on sperm motility, membrane integrity and mitochondrial function in red seabream ( Pagrus major ) sperm cryopreservation [Elektronische Daten] [Qinghua Liu, Xueying Wang, Wenqi Wang, Xuelei Zhang, Shihong Xu, Daoyuan Ma, Zhizhong Xiao, Yongshuang Xiao, Jun Li] The present study was to evaluate the effects of six antioxidants on frozen-thawed sperm motility, viability, membrane integrity and mitochondrial function in red seabream (Pagrus major) by computer-assisted sperm analysis system and flow cytometry, respectively. All the parameters tested in this study were determined using one-way ANOVA and identified using the SNK test (P<0.05). The results demonstrated that on the first day, the highest motility and longevity occurred in 100mM trehalose (78.34±3.41%, 29±4.00days) and 50mM taurine (77.46±1.54%, 29.33±4.04days), followed by 25mM vitamin C (79.03±5.37%, 17±1.00days), 25mM vitamin E (69.64±1.64%, 27.67±1.53days) and 25mM vitamin A (78.89±2.81%, 9.33±1.53days), which were all higher than frozen-thawed sperm without antioxidant (control) (66.80±5.55, 5.67±1.15days). Especially, the percentages of class A sperm with the addition of 100mM trehalose (40.39±5.20%) and 50mM taurine (37.78±3.22%) were significantly improved compared to the control (19.63±5.44%). The viability of all groups on the third and sixth day showed a similar trend. Moreover, during the 4°C storage process, the decrease of frozen-thawed sperm motility was closely associated with the decrease in membrane integrity and mitochondrial function. In conclusion, the present study indicated that antioxidant (100mM trehalose and 50mM taurine) provided the most pronounced protective effect in improving frozen-thawed quality of red seabream sperm. The addition of antioxidant may be capable of scavenging the ROS generated during the cryopreservation process and 4°C storage. Springer Science+Business Media Dordrecht, 2014 Red seabream nationallicence Cryopreservation nationallicence Sperm nationallicence Antioxidants nationallicence Liu Qinghua Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Wang Xueying Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Wang Wenqi College of Marine Science and Engineering, Qingdao Agricultural University, 266109, Qingdao, People's Republic of China aut Zhang Xuelei College of Marine Science and Engineering, Qingdao Agricultural University, 266109, Qingdao, People's Republic of China aut Xu Shihong Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Ma Daoyuan Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Xiao Zhizhong Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Xiao Yongshuang Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Li Jun Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 413-422 0920-1742 41:2<413 2015 41 10695 https://doi.org/10.1007/s10695-014-9993-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9993-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Qinghua Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xueying Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wenqi College of Marine Science and Engineering, Qingdao Agricultural University, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xuelei College of Marine Science and Engineering, Qingdao Agricultural University, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Xu Shihong Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Ma Daoyuan Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Zhizhong Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Yongshuang Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 700 1- Li Jun Center of Biotechnology R&D, Institute of Oceanology, Chinese Academy of Sciences, 266109, Qingdao, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 413-422 0920-1742 41:2<413 2015 41 10695