caa a22 4500 606230629 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-014-9991-y doi (NATIONALLICENCE)springer-10.1007/s10695-014-9991-y Identification and de novo sequencing of housekeeping genes appropriate for gene expression analyses in farmed maraena whitefish ( Coregonus maraena ) during crowding stress [Elektronische Daten] [Simone Altmann, Alexander Rebl, Carsten Kühn, Tom Goldammer] Maraena whitefish (Coregonus maraena; synonym Coregonus lavaretus f. balticus) is a high-quality food fish in the Southern Baltic Sea belonging to the group of salmonid fishes. Coregonus sp. is successfully kept in aquaculture throughout northern Europe (e.g. in Finland, Germany, Russia) and North America. In this regard, the molecular and immunological characterisation of stress response in maraena whitefish contributes to the development of robust and fast-growing maraena whitefish breeding strains for aquaculture. Thus, in the present study, the potential housekeeping genes beta actin (ACTB), elongation factor 1 alpha (EEF1A1), glyceraldehydes-3-phosphate dehydrogenase (GAPDH), ribosomal protein 9 (RPL9), ribosomal protein 32 (RPL32) and ribosomal protein S20 (RPS20) were de novo sequenced and tested concerning their applicability as reference genes in quantitative real-time PCR (qPCR) in maraena whitefish under different stocking densities. For this purpose, tissue samples of liver, kidney, gills, head kidney, skin, adipose tissue, heart and dorsal fin were investigated. qPCR data were analysed with Normfinder tool to determine gene expression stability. DNA sequencing exposed transcribed paralogous EEF1A1A and EEF1A1B genes differing in their putative protein structure. Normfinder analysis revealed RPL9 and RPL32 as most stable, GAPDH and ACTB as least stable genes for qPCR analyses, respectively. This is the first study that provides a subset of seven de novo sequenced housekeeping genes usable as reference genes in studies of stress response in maraena whitefish. Springer Science+Business Media Dordrecht, 2014 Coregonus maraena nationallicence ACTB nationallicence EEF1A1 nationallicence GAPDH nationallicence Housekeeping gene nationallicence RPL9 nationallicence RPL32 nationallicence RPS20 nationallicence Salmonidae nationallicence ACTB : Beta actin nationallicence BLAST : Basic local alignment search tool nationallicence EEF1A1 : Elongation factor 1 alpha nationallicence GAPDH : Glyceraldehydes-3-phosphate dehydrogenase nationallicence HSA : Homo sapiens nationallicence OMY : Oncorhynchus mykiss nationallicence RPL9 : Ribosomal protein 9 nationallicence RPL32 : Ribosomal protein 32 nationallicence RPS20 : Ribosomal protein S20 nationallicence qPCR : Quantitative real-time PCR nationallicence SSA : Salmo salar nationallicence Altmann Simone Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut Rebl Alexander Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut Kühn Carsten Institute for Fishery, State Research Institute for Agriculture and Fishing (LFA M-V), Born, Germany aut Goldammer Tom Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 397-412 0920-1742 41:2<397 2015 41 10695 https://doi.org/10.1007/s10695-014-9991-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-014-9991-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Altmann Simone Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut NATIONALLICENCE 700 1- Rebl Alexander Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut NATIONALLICENCE 700 1- Kühn Carsten Institute for Fishery, State Research Institute for Agriculture and Fishing (LFA M-V), Born, Germany aut NATIONALLICENCE 700 1- Goldammer Tom Fish Genetics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 397-412 0920-1742 41:2<397 2015 41 10695