caa a22 4500 606230637 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-015-0030-4 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0030-4 Molecular cloning and expression of two heat-shock protein genes (HSC70/HSP70) from Prenant's schizothoracin ( Schizothorax prenanti ) [Elektronische Daten] [Jiuxuan Li, Haibin Zhang, Xiuyue Zhang, Shiyong Yang, Taiming Yan, Zhaobin Song] Through the RT-PCR and rapid amplification of cDNA ends, two complementary deoxyribonucleic acid (cDNA) clones encoding heat-shock cognate 70 (HSC70, designated Sp-HSC70) and inducible heat-shock protein 70 (HSP70, designated Sp-HSP70) were isolated from the liver of Prenant's schizothoracin (Schizothorax prenanti). The cDNAs were 2344- and 2292-bp in length and contained 1950- and 1932-bp open reading frames, encoded proteins of 649 and 643 amino acids, respectively. Amino acid sequence analysis indicated that both Sp-HSC70 and Sp-HSP70 contained three signature sequences of HSP70 family, two partial overlapping bipartite nuclear localization signal sequences (an ATP-binding site motif, a bipartite nuclear targeting signal), and a cytoplasmic characteristic motif EEVD. Homology analysis revealed that Sp-HSC70 and Sp-HSP70 shared 77.5% identity and Sp-HSC70 shared more than 81.1% identity with the known HSC70s of other vertebrates, while Sp-HSP70 shared more than 77.5% identity with the known HSP70s of other vertebrates. Fluorescent real-time quantitative RT-PCR showed that Sp-HSC70 and Sp-HSP70 mRNAs were found in all tested tissues, including blood, brain, heart, liver, spleen, head kidney, white muscle, skin, gonad, hypophysis, red muscle, and gill. The Sp-HSC70 and Sp-HSP70 mRNA expression level in blood and head kidney displayed a significant increase in vibrio-challenged group with the bacterium Aeromonas hydrophila at 24h post-infection compared to a control group. Temporally, there was a clear time-dependent expression pattern of Sp-HSC70 or Sp-HSP70 gene after bacterial challenge, and the expression of Sp-HSC70 and Sp-HSP70 mRNAs reached a maximum level at 12 and 6h post-challenge, respectively. Both returned to control level after 7×24h. The results suggest that Sp-HSC70 and Sp-HSP70 genes may play important roles in mediating the immune responses of A. hydrophila-related diseases in the Prenant's schizothoracin. Springer Science+Business Media Dordrecht, 2015 Schizothorax prenanti nationallicence Heat-shock cognate 70 nationallicence Heat-shock protein 70 nationallicence Gene expression nationallicence Bacterial challenge nationallicence Li Jiuxuan Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut Zhang Haibin Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut Zhang Xiuyue Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut Yang Shiyong Department of Aquaculture, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Yan Taiming Department of Aquaculture, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Song Zhaobin Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 573-585 0920-1742 41:2<573 2015 41 10695 https://doi.org/10.1007/s10695-015-0030-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0030-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Jiuxuan Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Haibin Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiuyue Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Yang Shiyong Department of Aquaculture, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Yan Taiming Department of Aquaculture, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Song Zhaobin Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 573-585 0920-1742 41:2<573 2015 41 10695