caa a22 4500 606230653 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-015-0025-1 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0025-1 Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish ( Takifugu obscurus ) [Elektronische Daten] [Chang-Hong Cheng, Fang-Fang Yang, Shao-An Liao, Yu-Tao Miao, Chao-Xia Ye, An-Li Wang] Waterborne ammonia has become a persistent pollutant of aquatic habitats. The exposure to ammonia stress can reduce growth in a wide range of aquatic organisms. To assess the effect of ammonia exposure on the growth hormone/insulin-like growth factors (GH/IGF) axis, we identified and characterized GHR1, GHR2 and IGF-1 from pufferfish. Comparative analysis showed that these genes shared high identity and similarity with corresponding genes in other fish species. The transcripts of these genes were widely expressed in all tested tissues. The highest level of GHR1 mRNA was found in the brain, whereas GHR2 and IGF-1 mRNA levels were the highest in the liver. Following acute ammonia exposure (100mg/L total ammonia-nitrogen), GHR2 expression in the liver did not change at 6h and then significantly decreased at 12, 24 and 48h, whereas GHR1 and IGF-1 expressions were significantly down-regulated at 6, 12, 24 and 48h, respectively. These results indicated that ammonia stress decreased the expression of GH/IGF axis genes, which might have negative effect on the growth and development of pufferfish. Springer Science+Business Media Dordrecht, 2015 Pufferfish nationallicence Environmental stress nationallicence Cloning nationallicence Real-time PCR nationallicence Cheng Chang-Hong Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Yang Fang-Fang Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People's Republic of China aut Liao Shao-An Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Miao Yu-Tao Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Ye Chao-Xia Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Wang An-Li Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 495-507 0920-1742 41:2<495 2015 41 10695 https://doi.org/10.1007/s10695-015-0025-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0025-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Chang-Hong Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yang Fang-Fang Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liao Shao-An Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Miao Yu-Tao Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Ye Chao-Xia Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang An-Li Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, 510631, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 495-507 0920-1742 41:2<495 2015 41 10695