caa a22 4500 606230661 CHVBK 20210128101215.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10695-015-0026-0 doi (NATIONALLICENCE)springer-10.1007/s10695-015-0026-0 Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression [Elektronische Daten] [Arpana Rawat, Radha Chaube, Keerikkattil Joy] Vasotocin (VT) is the ortholog of vasopressin (VP) in non-mammalian vertebrates and is known for multiple functions. Teleost fishes have a complete repertoire of known VP/VT receptor subtypes (vasopressin type, VR): two V1A subtypes (V1Aa and V1Ab or V1a1 and V1a2) and five V2 subtypes (V2A1, V1A2, V2B1, V2B2 and V2C). Full-length cDNAs of v1a1, v1a2 and v2 (v2a1) with ORFs of 1,308, 1,137 and 1,527bp, respectively, were cloned and characterized in the catfish Heteropneustes fossilis (Siluriformes, Ostariophysi). BLAST analysis revealed that the genes encoded three VT receptors, V1a1, V1a2 and V2 of 436, 379 and 509 amino acid residues, respectively. The predicted proteins showed typical features of the seven-transmembrane domain receptor core structure with hallmark triplets Asp-Arg-Tyr/Asp-Arg-His (DRY/DRH) and the variable intracellular loop III of vertebrate neurohypophysial hormone receptors. Phylogenetic analysis of the deduced protein sequences revealed that they clustered with the V1Aa, V1Ab and V2A1, respectively, of other teleosts. The V2R has a sequence identity of 70-76% with V2A1 than with the V2B type (sequence identity 43-49%). Semiquantitative PCR analysis showed that the receptor gene transcripts were expressed ubiquitously in the tissues examined (brain, pituitary, gonads, liver, muscle, kidney and gills) and displayed sex and seasonal fluctuations in a tissue-specific manner. The results form a basis for functional studies on the VT receptors in the catfish. Springer Science+Business Media Dordrecht, 2015 Catfish nationallicence Phylogeny nationallicence Sex and seasonal expression nationallicence Vasotocin receptor genes nationallicence Rawat Arpana Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut Chaube Radha Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut Joy Keerikkattil Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 509-532 0920-1742 41:2<509 2015 41 10695 https://doi.org/10.1007/s10695-015-0026-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10695-015-0026-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rawat Arpana Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Chaube Radha Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 700 1- Joy Keerikkattil Department of Zoology, Centre of Advanced Study, Banaras Hindu University, 221005, Varanasi, India aut NATIONALLICENCE 773 0- Fish Physiology and Biochemistry Springer Netherlands 41/2(2015-04-01), 509-532 0920-1742 41:2<509 2015 41 10695