caa a22 4500 606233547 CHVBK 20210128101232.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2367-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2367-5 Extracellular galectin-3 induces MMP9 expression by activating p38 MAPK pathway via lysosome-associated membrane protein-1 (LAMP1) [Elektronische Daten] [Manohar Dange, Akhil Agarwal, Rajiv Kalraiya] Matrix metalloproteinases (MMPs) play a key role in matrix remodelling and thus invasion and metastasis. Extracellular galectin-3 has been shown to induce MMP9 secretion. Here, we demonstrate that galectin-3 induces MMP9 at transcript level and it is dependent on the surface levels of poly-N-acetyllactosamine (polyLacNAc). By employing signalling pathway inhibitors, MMP9 expression was shown to be induced via p38 MAP-kinase pathway. Using clones of melanoma cells expressing shRNAs to lysosome-associated membrane protein-1 (LAMP1), a major carrier of polyLacNAc, surface LAMP1 was demonstrated to serve as one of the key mediators of galectin-3-induced MMP9 expression via p38 MAPK pathway. Springer Science+Business Media New York, 2015 Galectin-3 nationallicence PolyLacNAc nationallicence MMP9 nationallicence p38 MAPK nationallicence LAMP1 nationallicence Dange Manohar Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut Agarwal Akhil Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut Kalraiya Rajiv Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 79-86 0300-8177 404:1-2<79 2015 404 11010 https://doi.org/10.1007/s11010-015-2367-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2367-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dange Manohar Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut NATIONALLICENCE 700 1- Agarwal Akhil Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut NATIONALLICENCE 700 1- Kalraiya Rajiv Kalraiya Lab, KS 131, Advanced Centre for Treatment Research and Education in Cancer (ACTREC), Tata Memorial Centre, Sector 22, 410210, Kharghar, Navi Mumbai, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 79-86 0300-8177 404:1-2<79 2015 404 11010