caa a22 4500 606233571 CHVBK 20210128101232.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2359-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2359-5 Association of myeloperoxidase polymorphism (G463A) with cervix cancer [Elektronische Daten] [Cindy Castelão, Alda da Silva, Andreia Matos, Ângela Inácio, Manuel Bicho, Rui Medeiros, Maria Bicho] Cervical cancer is the fourth most common cancer affecting women worldwide, according to the latest IARC release with 528 000 new cases every year. Infection by high-risk human papillomavirus (HPV) is necessary but not sufficient for progression to cancer. Epithelial tissues, the target of HPV infection, are heavily exposed to reactive oxygen species (ROS). Hypochlorous acid (HOCl) is a very potent ROS, and it is produced by myeloperoxidase (MPO). MPO, a lysosomal enzyme expressed in polymorphonuclear neutrophils (PMN), has the potential to kill HPV transformed cells, as a component of an intercellular induced-apoptosis pathway. This enzyme catalyzes the production of HOCl in the presence of hydrogen peroxide (H2O2). The H2O2 produced by the Doderlein's bacillus will interact with MPO, contributing to the intercellular induced-apoptosis pathway. We studied a functional polymorphism in the promoter region of MPO (G463A) and how it may affect the risk of developing cervix cancer. A sample of 100 patients with invasive cervical cancer and 122 control women were genotyped for MPO polymorphism by PCR-RFLP method. The statistical method used was χ2. We found that women with the GG genotype had lower risk for cervical cancer than the women who displayed the heterozygous genotype GA (OR=0.546, 95% CI=0.315-0.939, p=0.028, OR=2.210, 95% CI=1.257-3.886, p=0.008, respectively). The genotype that leads to a higher concentration of ROS (GG) presents itself as a protection factor in comparison to the homozygous genotype (AA). This can be explained by the interaction of HOCl and superoxide of transformed cells that will generate apoptosis-inducing hydroxyl radicals. Springer Science+Business Media New York, 2015 Cervical cancer nationallicence Oxidative stress nationallicence MPO nationallicence Vaginal microbiota nationallicence Polymorphism nationallicence Castelão Cindy Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut da Silva Alda Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut Matos Andreia Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut Inácio Ângela Faculty of Sciences, University of Lisbon, Lisbon, Portugal aut Bicho Manuel Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut Medeiros Rui Molecular Oncology Group, Portuguese Institute of Oncology Porto Centre, Porto, Portugal aut Bicho Maria Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 1-4 0300-8177 404:1-2<1 2015 404 11010 https://doi.org/10.1007/s11010-015-2359-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2359-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castelão Cindy Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut NATIONALLICENCE 700 1- da Silva Alda Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut NATIONALLICENCE 700 1- Matos Andreia Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut NATIONALLICENCE 700 1- Inácio Ângela Faculty of Sciences, University of Lisbon, Lisbon, Portugal aut NATIONALLICENCE 700 1- Bicho Manuel Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut NATIONALLICENCE 700 1- Medeiros Rui Molecular Oncology Group, Portuguese Institute of Oncology Porto Centre, Porto, Portugal aut NATIONALLICENCE 700 1- Bicho Maria Genetics Laboratory and Environmental Health Institute, Faculty of Medicine, University of Lisbon, Avenida Prof. Egas Moniz, 1649-028, Lisbon, Portugal aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 1-4 0300-8177 404:1-2<1 2015 404 11010