caa a22 4500 60623358X CHVBK 20210128101232.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2364-8 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2364-8 Hydrogen sulfide decreases the plasma lipid peroxidation induced by homocysteine and its thiolactone [Elektronische Daten] [Beata Olas, Bogdan Kontek] Hydrogen sulfide (H2S) has been investigated widely in recent years. H2S plays a variety of roles in different biological systems, including cardiovascular system. It is the final product of amino acids metabolism, which contains sulfur—cysteine and homocysteine (Hcy). In human plasma, there are several various forms of homocysteine: free Hcy, protein-bound Hcy (S-linked, and N-linked), and homocysteine thiolactone (HTL). Our previous works have shown that both Hcy in the reduced form and its thiolactone may modify fibrinolysis, coagulation process, and biological activity of blood platelets. Moreover, we have observed that HTL, like its precursor—Hcy stimulated the generation of superoxide anion radicals (O 2 −• ) in blood platelets. The aim of our study in vitro was to establish the influence of sodium hydrosulfide (NaHS, as a fast-releasing H2S donor; at tested concentrations: 10-1000µM) on the plasma lipid peroxidation induced by the reduced Hcy (at final concentrations of 0.01-1mM) and HTL (at final concentrations of 0.1-1µM). Our results indicate that 10 and 100µM NaHS decreased the lipid peroxidation in plasma treated with 1mM Hcy or 1µM HTL (when NaHS and Hcy/HTL were added to plasma together). The protective effect of 10 and 100µM NaHS against the lipid peroxidation in plasma preincubated with 1mM Hcy or 1µM HTL was also observed. Considering the data presented in this study, we suggest that the lipid peroxidation (induced by different forms of homocysteine) may be reduced by hydrogen sulfide. Springer Science+Business Media New York, 2015 Homocysteine nationallicence Homocysteine thiolactone nationallicence Plasma nationallicence Lipid peroxidation nationallicence Hydrogen sulfide nationallicence Olas Beata The Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut Kontek Bogdan The Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 39-43 0300-8177 404:1-2<39 2015 404 11010 https://doi.org/10.1007/s11010-015-2364-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2364-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Olas Beata The Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut NATIONALLICENCE 700 1- Kontek Bogdan The Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 39-43 0300-8177 404:1-2<39 2015 404 11010