caa a22 4500 606233598 CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2381-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2381-7 Decrease of serum adenine nucleotide hydrolysis in an irritant contact dermatitis mice model: potential P2X7R involvement [Elektronische Daten] [R. Zanin, G. da Silva, T. Erig, N. Sperotto, C. Leite, R. Coutinho-Silva, A. Batastini, Fernanda Morrone] Extracellular adenosine 5′-triphosphate (ATP) has significant effects on a variety of pathological conditions and it is the main physiological agonist of P2X7 purinergic receptor (P2X7R). It is known that ATP acting via purinergic receptors plays a relevant role on skin inflammation, and P2X7R is required to neutrophil recruitment in a mice model of irritant contact dermatitis (ICD).The present study investigated the effects of chemical irritant croton oil (CrO) upon ATP, ADP, and AMP hydrolysis in mice blood serum, and the potential involvement of P2X7R. The topical application CrO induced a decrease on soluble ATP/ADPase activities (~50%), and the treatment with the selective P2X7R antagonist, A438079, reversed these effects to control level. Furthermore, we showed that CrO decreased cellular viability (52.6%±3.9) in relation to the control and caused necrosis in keratinocytes (PI positive cells). The necrosis induced by CrO was prevented by the pre-treatment with the selective P2X7R antagonist A438079. The results presented herein suggest that CrO exerts an inhibitory effect on the activity of ATPDase in mouse serum, reinforcing the idea that ICD has a pathogenic mechanism dependent of CD39. Furthermore, it is tempting to suggest that P2X7R may act as a controller of the extracellular levels of ATP. Springer Science+Business Media New York, 2015 P2X7R nationallicence Keratinocytes nationallicence ATP nationallicence Nucleotide hydrolysis nationallicence Contact dermatitis nationallicence Zanin R. Programa de Pós-Graduação em Biologia Celular e Molecular, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut da Silva G. Programa de Pós-Graduação em Biologia Celular e Molecular, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut Erig T. Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut Sperotto N. Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut Leite C. Instituto de Toxicologia e Farmacologia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut Coutinho-Silva R. Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil aut Batastini A. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil aut Morrone Fernanda Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 221-228 0300-8177 404:1-2<221 2015 404 11010 https://doi.org/10.1007/s11010-015-2381-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2381-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zanin R. Programa de Pós-Graduação em Biologia Celular e Molecular, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- da Silva G. Programa de Pós-Graduação em Biologia Celular e Molecular, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Erig T. Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Sperotto N. Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Leite C. Instituto de Toxicologia e Farmacologia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Coutinho-Silva R. Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Batastini A. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Morrone Fernanda Faculdade de Farmácia, Pontificia Universidade Catolica do Rio Grande do Sul - PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 221-228 0300-8177 404:1-2<221 2015 404 11010