caa a22 4500 60623361X CHVBK 20210128101233.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11010-015-2375-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2375-5 MAPKs' status at early stages of renal carcinogenesis and tumors induced by ferric nitrilotriacetate [Elektronische Daten] [Francisco Aguilar-Alonso, José Solano, Chabetty Vargas-Olvera, Ignacio Pacheco-Bernal, Telma Pariente-Pérez, María Ibarra-Rubio] Renal cell carcinoma (RCC) is asymptomatic at early stages, and thus, initial diagnosis frequently occurs at advanced or even metastatic stages, leading to a high rate of mortality. Ferric nitrilotriacetate (FeNTA)-induced RCC model is a useful tool to analyze molecular events at different stages of the carcinogenesis process in vivo. MAPKs' alterations seem to play an important role in the development and maintenance of human RCC tumors. Based on the above, p38α/β/γ, JNK1/2, and ERK1/2 statuses were studied at early stages of FeNTA-induced renal carcinogenesis (1 and 2months of carcinogen treatment) as well as in tumor tissue. MAPKs showed distinct response along carcinogenesis process, either as total proteins and/or as their phosphorylated forms. While the increase in total and phospho-p38α/β levels became lower as carcinogenesis progressed, p38γ overexpression grew. Instead, total JNK2 diminished, but JNK1 was elevated at all studied times, and p-JNK1 levels increased at early stages, but not in tumors. In contrast, p-JNK2 rose at 2months of treatment and in tumor tissue. Increased levels of p-ERK1/2 were observed at all stages analyzed. Very interestingly, at 1 and 2months of FeNTA treatment, no alterations in MAPKs were found in liver or lung, where no primary tumors are induced with the scheme of FeNTA administration followed here. In conclusion, MAPKs' behavior evolved differentially as renal carcinogenesis advanced, even among isoforms of the same family, but it did not change in other tissues. All this strongly suggests a role of these kinases in FeNTA-induced RCC tumor development and maintenance. Springer Science+Business Media New York, 2015 Renal cell carcinoma nationallicence Ferric nitrilotriacetate nationallicence MAPK nationallicence Carcinogenesis nationallicence Aguilar-Alonso Francisco Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Solano José Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Vargas-Olvera Chabetty Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Pacheco-Bernal Ignacio Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Pariente-Pérez Telma Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Ibarra-Rubio María Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 161-170 0300-8177 404:1-2<161 2015 404 11010 https://doi.org/10.1007/s11010-015-2375-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2375-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aguilar-Alonso Francisco Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Solano José Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Vargas-Olvera Chabetty Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Pacheco-Bernal Ignacio Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Pariente-Pérez Telma Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 700 1- Ibarra-Rubio María Laboratorio F-120, Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, 04510, Mexico, DF, Mexico aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 404/1-2(2015-06-01), 161-170 0300-8177 404:1-2<161 2015 404 11010